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Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
Published on: December 23, 2015
Las "células progenitoras endoteliales" de la sangre periférica se derivan de monocitos/macrófagos y secretan
Jalees Rehman1, Jingling Li, Christie M Orschell
1Krannert Institute of Cardiology, Department of Medicine, Indiana University School of Medicine, Indianapolis, USA.
Circulation
|March 5, 2003
Resumen
Las células progenitoras endoteliales (EPC) no proliferan, sino que secretan factores de crecimiento angiogénico, que actúan principalmente a través de estos factores en lugar de la diferenciación endotelial directa. Estos hallazgos sugieren nuevas estrategias terapéuticas para promover el crecimiento de los vasos sanguíneos.
Área de la Ciencia:
- Biología celular Biología celular.
- Investigación de la angiogénesis Investigación de la angiogénesis
- Inmunología Inmunología.
Sus antecedentes:
- Las células progenitoras endoteliales (EPC) de la sangre periférica pueden mejorar la angiogénesis.
- El mecanismo de la angiogénesis mediada por EPC (diferenciación endotelial frente a la secreción de factor de crecimiento) sigue sin estar claro.
Objetivo del estudio:
- Para investigar el mecanismo de los efectos proangiogénicos mediados por EPC.
- Para determinar si las EPC proliferan y diferencian o secretan factores angiogénicos.
Principales métodos:
- EPCs humanos aislados y fenotipo confirmado utilizando captación de LDL acetilado y unión a la ulex-lectina.
- Se analizó la proliferación de EPC y los marcadores superficiales mediante citometría de flujo.
- Medio acondicionado ensayado para factores de crecimiento angiogénico.
Principales resultados:
- Las EPC aisladas son predominantemente marcadores de monocitos/macrófagos expresados (CD14, Mac-1, CD11c) con un mínimo de marcadores endoteliales (VE-cadherina) o de células madre (AC133, c-kit).
- Los EPC cultivados mostraron una activación monocítica regulada y marcadores de diferenciación de macrófagos.
- Las EPC no exhibieron una proliferación significativa, pero secretaron factores angiogénicos clave (VEGF, HGF, G-CSF, GM-CSF).
Conclusiones:
- Las células acetil-LDL (((+) ulex-lectina (((+) (comúnmente llamadas EPC) funcionan principalmente a través de la secreción de factores de crecimiento proangiogénicos, no a través de la proliferación o la diferenciación endotelial significativa.
- Estas células se derivan en gran medida de los monocitos y macrófagos.
- Es probable que los efectos angiogénicos estén mediados por factores secretados, lo que sugiere un potencial terapéutico en las estrategias basadas en el factor de crecimiento o el reclutamiento de monocitos para el tratamiento de la isquemia.
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