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Published on: May 30, 2017
Secuencia del genoma de Vibrio parahaemolyticus: un mecanismo patógeno distinto al de V cholerae
Kozo Makino1, Kenshiro Oshima, Ken Kurokawa
1Department of Molecular Microbiology, Osaka University, Osaka, Japan.
Lancet (London, England)
|March 7, 2003
Resumen
Vibrio parahaemolyticus causa gastroenteritis y posee genes del sistema de secreción de tipo III (TTSS), a diferencia de V. cholerae. Esta diferencia genómica explica los distintos mecanismos de infección y los síntomas clínicos entre estas bacterias relacionadas.
Área de la Ciencia:
- Microbiología Microbiología.
- La genómica es la genómica.
- Enfermedades infecciosas Enfermedades infecciosas.
Sus antecedentes:
- Vibrio parahaemolyticus es una bacteria marina Gram-negativa responsable de la gastroenteritis global transmitida por los alimentos.
- Serotipos específicos de V. parahaemolyticus han causado una pandemia, y el organismo está estrechamente relacionado con Vibrio cholerae.
Objetivo del estudio:
- Para determinar la secuencia del genoma completo de una cepa clínica de V. parahaemolyticus.
- Para comparar el genoma de V. parahaemolyticus con el de V. cholerae para identificar diferencias genéticas.
- Investigar el papel de factores genéticos específicos en la patogenicidad distintiva de V. parahaemolyticus.
Principales métodos:
- Secuenciación del genoma completo de la cepa de V. parahaemolyticus RIMD2210633 utilizando secuenciación de escopeta.
- Identificación de secuencias de codificación utilizando los programas Gambler y Glimmer.
- Análisis genómico comparativo con V. cholerae utilizando MUMmer.
Principales resultados:
- El genoma de V. parahaemolyticus comprende dos cromosomas circulares (3.288.558 bp y 1.877.212 bp) con 4.832 genes.
- Se observaron reordenamientos genómicos significativos entre los cromosomas de V. parahaemolyticus y V. cholerae.
- Los genes que codifican el sistema de secreción de tipo III (TTSS) fueron identificados en V. parahaemolyticus pero ausentes en V. cholerae.
Conclusiones:
- El sistema de secreción de tipo III (TTSS) es un factor de virulencia clave en las bacterias que causan gastroenteritis.
- V. parahaemolyticus y V. cholerae emplean diferentes estrategias para la infección.
- La presencia de TTSS en V. parahaemolyticus explica su diarrea inflamatoria y su potencial para infecciones sistémicas, diferenciándola de la diarrea no inflamatoria de V. cholerae.
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