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Segregación funcional y espacial de las piscinas de vesículas secretoras según la edad de las vesículas

Rory R Duncan¹, Jennifer Greaves, Ulrich K Wiegand

¹Membrane Biology Group, University of Edinburgh, George Square, Edinburgh EH8 9XD, UK.

|March 14, 2003

Resumen

Las vesículas secretoras no se reclutan al azar para la exocitosis. Las nuevas vesículas atracan en la membrana plasmática, mientras que las vesículas más antiguas forman un grupo citoplasmático móvil, con secretagogues que se liberan selectivamente de cualquiera de los grupos.

Área de la Ciencia:

Biología celular Biología celular.
La neuroendocrinología en neuroendocrinología.
La bioquímica es la bioquímica.

Sus antecedentes:

Las terminales sinápticas y las células neuroendocrinas utilizan vesículas secretoras para la exocitosis.
Un pequeño grupo fácilmente liberable (RRP) de vesículas está acoplado a la membrana plasmática, mientras que un grupo de reserva más grande reside en el citoplasma.
Actualmente se desconoce el mecanismo para priorizar el reclutamiento de vesículas en el RRP.

Objetivo del estudio:

Para investigar la segregación dependiente de la edad y el reclutamiento de las vesículas secretoras en las células de cromafina suprarrenal bovina.
Determinar si la edad de las vesículas influye en su distribución espacial y accesibilidad para la exocitosis.

Principales métodos:

Utilizó una proteína fluorescente de carga que cambia de color con el tiempo para rastrear la edad de las vesículas.
Comportamiento y distribución de las vesículas observadas en las células de cromafina suprarrenal bovina.
Se aplicaron diferentes secretagógicos para evaluar la liberación selectiva de las vesículas.

Principales resultados:

Las vesículas se segregan en poblaciones distintas basadas en la edad.
Las vesículas recién ensambladas son inmóviles y acopladas a la membrana plasmática.
Las vesículas más viejas son móviles y se encuentran más profundamente dentro del citoplasma.
Los secretagogues liberan selectivamente las vesículas ya sea de la RRP o de la piscina citoplasmática más profunda.

Conclusiones:

El reclutamiento de vesículas para la exocitosis no es aleatorio, sino que está influenciado por la edad de la vesícula.
Las vesículas se segregan funcional y espacialmente según su edad, lo que afecta a su dinámica de liberación.
Esta segregación basada en la edad proporciona un nuevo mecanismo para regular la exocitosis.

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