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Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Impacto de las propiedades físicas del líquido iónico en la actividad de la lipasa y la estabilidad
Joel L Kaar1, Anita M Jesionowski, Jason A Berberich
1McGowan Institute for Regenerative Medicine and Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Journal of the American Chemical Society
|April 3, 2003
Resumen
Se exploró la actividad y estabilidad de la enzima lipasa en varios líquidos iónicos. Mientras que algunos líquidos iónicos aumentaron la actividad de la lipasa, otros, particularmente los hidrófilos, no mostraron ninguna función enzimática detectable.
Área de la Ciencia:
- La biocatálisis por biocatálisis.
- Ingeniería de enzimas Ingeniería de enzimas Ingeniería de enzimas
- Los líquidos iónicos son líquidos iónicos.
Sus antecedentes:
- Los líquidos iónicos (IL) ofrecen propiedades únicas de disolvente para las reacciones enzimáticas.
- Investigar el rendimiento de la lipasa en las IL es crucial para desarrollar nuevos procesos biocatalíticos.
Objetivo del estudio:
- Para evaluar la actividad de la lipasa y la estabilidad en diversos líquidos iónicos a base de dialquillimidazolio y pirrolidinio.
- Para comparar el rendimiento de IL con los disolventes orgánicos convencionales para la transesterificación enzimática y la politransesterificación.
Principales métodos:
- Tasas iniciales evaluadas de transesterificación del metacrilato de metilo catalizado por la Candida rugosa lipasa libre.
- Se examinó la politransesterificación del adipado de divinyl y el 1,4-butanediol utilizando Novozym 435.
- Se han investigado técnicas de estabilización enzimática (adsorción, modificación de PEG, inmovilización).
- Realizó estudios solvatocrómicos y mediciones del coeficiente de partición para caracterizar las propiedades de IL.
Principales resultados:
- La actividad de la lipasa fue 1,5 veces mayor en el hexafluorofosfato de 1-butilo-3-metilimidazolio en comparación con el hexano.
- No se observó actividad de lipasa detectable en líquidos iónicos hidrófilos.
- Los métodos de estabilización enzimática no lograron mejorar la actividad de las ILs hidrófilas.
- Los pesos moleculares del poliéster se limitaron a 2900 Da en el hexafluorofosfato de 1-butilo-3-metilimidazolio debido a la precipitación del polímero.
- Las lipasas demostraron una mayor estabilidad en las ILs en comparación con los disolventes orgánicos.
Conclusiones:
- Los líquidos iónicos específicos, como el hexafluorofosfato de 1-butilo-3-metilimidazolio, pueden mejorar las reacciones catalizadas por lipasa.
- Los líquidos iónicos hidrófilos no son adecuados para las reacciones catalizadas por lipasa estudiadas.
- Los líquidos iónicos ofrecen una estabilidad enzimática mejorada que los disolventes orgánicos tradicionales.
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