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Diferenciación de células humanas derivadas de la médula ósea en células epiteliales bucales in vivo: un estudio
Simon D Tran1, Stanley R Pillemer, Amalia Dutra
1National Institute of Dental and Craniofacial Research, Gene Therapy and Therapeutics Branch National Institutes of Health, Bethesda, MD 20892-1190, USA. stran@dir.nidcr.nih.gov <stran@dir.nidcr.nih.gov>
Lancet (London, England)
|April 4, 2003
Resumen
Las células adultas derivadas de la médula ósea pueden transformarse en células epiteliales in vivo sin fusionarse con las células receptoras. Esto demuestra una significativa plasticidad celular, ofreciendo potencial para aplicaciones de medicina regenerativa.
Área de la Ciencia:
- Biología de las células madre Biología de las células madre
- La medicina regenerativa es una medicina regenerativa.
- Plasticidad celular La plasticidad celular.
Sus antecedentes:
- Las células adultas derivadas de la médula ósea (BMD) muestran potencial para la reparación de tejidos.
- Los estudios in vitro plantearon preguntas sobre la plasticidad celular de la DMO, sugiriendo la fusión celular en lugar de la diferenciación.
- El papel in vivo de la fusión celular en la diferenciación celular de la DMO sigue sin estar claro.
Objetivo del estudio:
- Investigar si las células derivadas de la médula ósea pueden colonizar los tejidos epiteliales y diferenciarse sin fusión.
- Para determinar la importancia in vivo de los eventos de fusión en la plasticidad de las células madre adultas.
Principales métodos:
- Se utilizó la hibridación in situ con sondas de cromosomas Y y X (etiquetadas con 35-azufre, digoxigenina o fluorescente).
- Se realizó inmunohistoquímica con anticitoqueratina 13.
- Se analizaron células epiteliales bucales de mujeres que habían recibido trasplantes de médula ósea masculina o de células madre de sangre periférica.
Principales resultados:
- Se detectaron células bucales positivas para el cromosoma Y en todas las mujeres receptoras 4-6 años después del trasplante (0.8-12.7%).
- Muy pocas células (0,01%) mostraron eventos potenciales de fusión (células XY+XX).
- Las células de DMO masculinas migraron y se diferenciaron con éxito en células epiteliales bucales en receptores femeninas.
Conclusiones:
- Las células masculinas derivadas de la médula ósea migran a la mejilla y se diferencian en células epiteliales sin fusionarse con las células receptoras.
- Este proceso puede representar la transdiferenciación de las células progenitoras hematopoyéticas o estomales.
- La plasticidad de las células de DMO es prometedora para las terapias de medicina regenerativa.
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