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La regulación de la ARN polimerasa II alargada por los factores de transcripción de la división en las levaduras

Antonin Morillon¹, Justin O'Sullivan, Abul Azad

¹Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.

Science (New York, N.Y.)

|April 19, 2003

Resumen

Los factores de transcripción de la horquilla de la levadura Fkh1p y Fkh2p regulan el alargamiento y la terminación de la transcripción génica. Estos factores coordinan el alargamiento temprano de la transcripción con el procesamiento del ARN mensajero, lo que afecta la regulación de los genes eucariotas.

Área de la Ciencia:

Biología Molecular Biología Molecular
Regulación genética Reglamento genético.
La bioquímica es la bioquímica.

Sus antecedentes:

El alargamiento de la transcripción por la ARN polimerasa II (RNAPII) es un proceso regulado.
Los puntos de control de alargamiento temprano vinculan la transcripción al procesamiento del ARN pre-mensajero (ARNm previo).
El dominio carboxiterminal (CTD) del RNAPII se somete a fosforilación durante la transcripción.

Objetivo del estudio:

Investigar el papel de los factores de transcripción Fkh1p y Fkh2p en la regulación de la transcripción.
Determinar cómo Fkh1p y Fkh2p influyen en el alargamiento del RNAPII y en el procesamiento del pre-ARNm.
Explorar la coordinación entre el alargamiento de la transcripción y la fosforilación de la CTD.

Principales métodos:

Asociación de Fkh1p y Fkh2p con regiones codificantes de genes activos.
Análisis de la elongación y terminación de la transcripción.
Monitoreo de la fosforilación de serina-5 y serina-2 del CTD RNAPII.

Principales resultados:

Fkh1p y Fkh2p se asocian con las regiones codificantes de los genes activos.
Estos factores influyen de manera opuesta en el alargamiento y la terminación de la transcripción.
Los eventos mediados por Fkh1p/Fkh2p están coordinados con la fosforilación de RNAPII CTD en serina-5 y -2.

Conclusiones:

Fkh1p y Fkh2p, más allá de las funciones de promotor, coordinan el alargamiento temprano de la transcripción y el procesamiento pre-ARNm.
Esta coordinación está vinculada a los patrones específicos de fosforilación de la CTD RNAPII.
Los hallazgos sugieren un mecanismo general para la regulación génica en los eucariotas.

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