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Distribución específica del programa de un factor de transcripción dependiente del factor de transcripción asociado y
Julia Zeitlinger1, Itamar Simon, Christopher T Harbison
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA. zeitlinger@wi.mit.edu
Cell
|May 7, 2003
Resumen
El factor de transcripción de la levadura Ste12 se une a diferentes genes basados en señales de desarrollo, no en un interruptor global. Esta unión dependiente de la condición, regulada por las cinasas Tec1 y MAP, sugiere un mecanismo general para controlar los programas de expresión génica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética de la levadura Genética de la levadura
- El Reglamento de transcripción.
Sus antecedentes:
- Las vías de señalización activan los factores de transcripción para inducir programas especializados de expresión génica.
- El mecanismo por el cual los factores de transcripción regulan múltiples programas de desarrollo sigue sin estar claro.
- Se desconoce si los factores de transcripción cambian globalmente la selección de promotores para diferentes funciones.
Objetivo del estudio:
- Investigar si el factor de transcripción de la levadura Ste12 funciona en múltiples programas de desarrollo a través de un interruptor global en la selección de promotores.
- Para determinar la unión dependiente de la condición de Ste12 a los genes objetivo.
- Para aclarar los mecanismos regulatorios que controlan la distribución específica del programa de Ste12.
Principales métodos:
- El análisis de localización de todo el genoma (ChIP-chip o similar) se empleó para mapear los sitios de unión de Ste12.
- Investigó el papel del factor de transcripción Tec1 en la unión de Ste12 durante el crecimiento filamentoso.
- Se examinó la regulación diferencial de Ste12 por las cinasas MAP Fus3 y Kss1.1.
Principales resultados:
- Ste12 se une a conjuntos distintos de genes objetivo dependiendo de la condición específica de desarrollo (acoplamiento versus crecimiento filamentoso).
- La distribución dependiente de las condiciones de Ste12 requiere la unión concurrente de Tec1 durante el crecimiento filamentoso.
- Las quinasas MAP Fus3 y Kss1 regulan diferencialmente la actividad y la unión de Ste12.
Conclusiones:
- Ste12 no utiliza un interruptor promotor global, sino que exhibe una unión dependiente de la condición a genes específicos del programa.
- La distribución genómica específica del programa es un potencial mecanismo general para que los factores de transcripción regulen distintos programas de expresión génica.
- Este hallazgo proporciona información sobre la intrincada regulación de la diferenciación celular y la respuesta a las vías de señalización.
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