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Facilitated Transport01:19

Facilitated Transport

The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In facilitated transport, also known as facilitated diffusion, molecules and ions travel across a membrane via...
Facilitated Transport01:19

Facilitated Transport

The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a membrane via...
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In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
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Video Experimental Relacionado

Updated: Jul 8, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

Un canal acuoso multifuncional formado por el CFTR.

H Hasegawa1, W Skach, O Baker

  • 1Department of Medicine, University of California, San Francisco 94143-0532.

Science (New York, N.Y.)
|November 27, 1992
PubMed
Resumen
Este resumen es generado por máquina.

La proteína del regulador de conductividad transmembrana de la fibrosis quística (CFTR) facilita el transporte estimulado por la cAMP de aniones, agua y urea. Este estudio confirma que la CFTR contiene un poro acuoso funcional, lo que proporciona evidencia del movimiento del agua a través de canales iónicos.

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Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Fisiología Fisiología Fisiología.
  • La biofísica es la biofísica.

Sus antecedentes:

  • El producto del gen de la fibrosis quística (CFTR) es un canal iónico estimulado por el cAMP.
  • Su papel en la regulación de los procesos intracelulares y su potencial para el transporte acuoso no se comprenden completamente.

Objetivo del estudio:

  • Para determinar si la molécula CFTR posee una vía acuosa funcional.
  • Para investigar el transporte de aniones, agua y urea a través de la CFTR.

Principales métodos:

  • Los ensayos de transporte se realizaron utilizando ovocitos de Xenopus que expresan CFTR.
  • Las mediciones incluyeron la permeabilidad de aniones, agua y urea.
  • Se evaluaron los efectos de los agonistas de la cAMP y los bloqueadores de los canales Cl.

Principales resultados:

  • La estimulación de cAMP indujo una conductividad Cl significativa y un aumento de la permeabilidad al agua en los ovocitos que expresan CFTR.
  • La permeabilidad del agua dependía de la composición aniónica y era inhibida por un bloqueador del canal Cl-.
  • El CFTR demostró el transporte estimulado por el CAMP de urea pero no de sacarosa, lo que indica una vía parecida a un poro.

Conclusiones:

  • La CFTR funciona como un poro acuoso estimulado por la cAMP capaz de transportar aniones, agua y pequeños solutos como la urea.
  • Estos hallazgos proporcionan evidencia funcional para el movimiento del agua a través de un canal iónico.
  • El estudio aclara las capacidades de transporte de la proteína CFTR.