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La actividad de la ornitina descarboxilasa es crítica para la transformación celular
M Auvinen1, A Paasinen, L C Andersson
1Department of Pathology, University of Helsinki, Finland.
Nature
|November 26, 1992
Resumen
La ornithine decarboxylase (ODC) actúa como un proto-oncogén, impulsando la transformación y el crecimiento celular. La inhibición de ODC bloquea la transformación celular inducida por oncogenes, destacando su papel central en el desarrollo del cáncer.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- Oncología Oncología.
Sus antecedentes:
- Las poliaminas son cruciales para la proliferación celular, reguladas por la ornitina descarboxilasa (ODC).
- La expresión de ODC se eleva durante la transformación celular inducida por carcinógenos, virus u oncogenes.
Objetivo del estudio:
- Investigar si la ornitina descarboxilasa (ODC) media la transformación celular y posee propiedades oncogénicas.
- Para determinar el papel de la ODC en la transformación celular inducida por oncogenes.
Principales métodos:
- Transfección de células NIH3T3 con ADNc ODC humano en orientaciones de sentido y antisenso.
- Evaluar la transformación celular, el crecimiento independiente del anclaje y la fosforilación de la proteína tirosina.
- Inhibición de la ODC endógena y el uso de ARN antisense para bloquear la transformación por v-src oncogene.
Principales resultados:
- La sobreexpresión de ODC indujo la transformación celular, el crecimiento independiente del anclaje y la fosforilación de tirosina de una proteína 130K.
- La expresión de ARN ODC antisense correlacionada con la morfología epitelioide y la reducción de la proliferación.
- La inhibición de ODC o la síntesis de ARN antisense impidieron la transformación de fibroblastos inducida por oncogenes v-src.
Conclusiones:
- El gen de la ornitina descarboxilasa (ODC) funciona como un proto-oncogén.
- ODC juega un papel fundamental en la regulación del crecimiento y la transformación celular.
- Dirigirse a ODC puede ofrecer estrategias terapéuticas para el tratamiento del cáncer.
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