Video Experimental Relacionado
Updated: Jul 19, 2026
04:48
A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate
Published on: July 10, 2018
Necesidad de la fosforilación GluR2 del receptor AMPA para la depresión cerebelosa a largo plazo
Hee Jung Chung1, Jordan P Steinberg, Richard L Huganir
1Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 USA.
Science (New York, N.Y.)
|June 14, 2003
Resumen
La depresión cerebelosa a largo plazo (LTD), una forma de memoria sináptica, depende de la proteína quinasa C (PKC) para reducir los receptores postsinápticos de AMPA. La fosforilación por PKC de GluR2 en Ser880 es esencial para inducir la LTD cerebelosa.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- La plasticidad sináptica.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La depresión cerebelosa a largo plazo (LTD) es un mecanismo clave para la memoria sináptica en el cerebelo.
- LTD implica una disminución en los receptores postsinápticos de alfa-amino-3-hidroxi-5-metil-4-isoxazolpropionato (AMPA).
- La activación de la proteína quinasa C (PKC) es necesaria para la inducción de LTD.
Objetivo del estudio:
- Investigar el papel de la fosforilación de la subunidad GluR2 por PKC en el cerebelo LTD.
- Para determinar si los sitios específicos de fosforilación en GluR2 son críticos para la expresión de LTD.
Principales métodos:
- Se utilizaron células Purkinje cerebelares cultivadas de ratones mutantes que carecían de la subunidad GluR2.
- Transfección transitoria empleada con subunidades GluR2 de tipo salvaje y mutantes.
- Se evaluó el rescate y la oclusión de LTD por mutaciones específicas de GluR2.
Principales resultados:
- LTD estaba ausente en las células que carecían de la subunidad GluR2 y podría rescatarse mediante la transfección de GluR2 de tipo salvaje.
- La transfección con un mutante GluR2 que impide la fosforilación de PKC en Ser880 no pudo restaurar LTD.
- Un mutante de GluR2 que imita la fosforilación en Ser880 ocluyó la posterior LTD.
Conclusiones:
- La fosforilación por PKC de la subunidad GluR2 en Ser880 es un evento crítico para la inducción de LTD cerebeloso.
- Este evento de fosforilación es esencial para la reducción de los receptores de AMPA durante la LTD.
- Identifica un mecanismo molecular específico que subyace a la memoria sináptica en el cerebelo.
Videos de Conceptos Relacionados
01:35Long-term Potentiation
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
01:05Long-term Depression
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
01:25Long-term Potentiation
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when presynaptic neurons...
Hebbian LTP
LTP can occur when presynaptic neurons...
01:03Long-term Depression
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Calcium Ion Concentration Mechanism
If over time, all...
Calcium Ion Concentration Mechanism
If over time, all...
01:12GPCR Desensitization
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
01:30Ligand-Gated Ion Channel Receptor: Gating Mechanism
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...