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Extracellular Vesicle Tissue Factor Activity Assay
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El factor de activación plaquetaria acetilhidrolasa está asociado principalmente con la subfracción electronegativa
Sonia Benítez1, José Luis Sánchez-Quesada, Vicent Ribas
1Servei de Bioquímica and Institut de Recerca, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Circulation
|June 25, 2003
Resumen
El LDL electronegativo (LDL(-)) contiene una actividad significativamente mayor del factor de activación plaquetaria acetilhidrolasa (PAF-AH). Este aumento de PAF-AH en LDL (((-) puede contribuir a sus efectos inflamatorios en las células endoteliales.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- El metabolismo de los lípidos.
- Investigación Cardiovascular Investigación Cardiovascular Investigación Cardiovascular Investigación Cardiovascular Investigación Cardiovascular
Sus antecedentes:
- El LDL electronegativo (LDL(-)) es una fracción de lipoproteína modificada.
- LDL(-) induce la liberación de citoquinas proinflamatorias (IL-8, MCP-1) desde las células endoteliales.
Objetivo del estudio:
- Investigar la asociación del factor de activación plaquetaria acetilhidrolasa (PAF-AH) con el LDL electronegativo (LDL(-)).
- Para determinar si la actividad de PAF-AH difiere entre LDL ((-) y LDL no electronegativo (LDL ((+)).
Principales métodos:
- Se evaluó la actividad de PAF-AH en LDL ((-) y LDL ((+)) de sujetos con normolipemia e hipercolesterolemia.
- Se utilizó el análisis de Western blot y SDS-PAGE para identificar PAF-AH.
- Empleó electroforesis en gel de gradiente de poliacrilamida no naturalizante para evaluar la unión de PAF-AH a LDL.
Principales resultados:
- El PAF-AH se asoció predominantemente con el LDL, mostrando una actividad cinco veces mayor en comparación con el LDL.
- El Western blot confirmó la presencia de PAF-AH en el LDL, pero no en el LDL.
- Los ácidos grasos no esterificados, un producto de PAF-AH, se elevaron en el LDL.
Conclusiones:
- El aumento de la actividad de PAF-AH en LDL (((-) es un hallazgo clave.
- Esta alta actividad de PAF-AH puede explicar las propiedades pro-inflamatorias de la LDL (((-) en las células endoteliales.
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