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Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
Published on: June 27, 2017
Reclutamiento del proteasoma dependiente de Cks1 y activación de la transcripción CDC20 en levaduras en ciernes
May C Morris1, Peter Kaiser, Stanislav Rudyak
1The Scripps Research Institute, Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Nature
|June 27, 2003
Resumen
La proteína Cks1 promueve la mitosis en la levadura mediante la regulación de la transcripción de Cdc20. Ayuda a disociar la quinasa Cdc28 y recluta al proteosoma al promotor CDC20 para la expresión genética adecuada.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- Las proteínas Cks son proteínas conservadas que interactúan con las cinasas dependientes de ciclina, pero sus funciones siguen sin estar claras.
- La investigación existente en genética, bioquímica y biología estructural no ha producido un modelo unificado para las funciones de la proteína Cks.
Objetivo del estudio:
- Para investigar el papel esencial de Cks1 en Saccharomyces cerevisiae.
- Para aclarar el mecanismo por el cual Cks1 promueve la mitosis.
Principales métodos:
- Investigó el papel de Cks1 en Saccharomyces cerevisiae.
- Examinó la interacción de Cks1 con el promotor CDC20, la cinasa Cdc28 y el proteosoma.
Principales resultados:
- Cks1 es esencial para promover la mitosis.
- Cks1 modula la activación transcripcional de Cdc20, un activador de la APC/C proteína ubiquitina ligasa.
- Cks1 es necesario para la disociación periódica de la cinasa Cdc28 del promotor CDC20 y la asociación periódica del proteosoma con el promotor.
Conclusiones:
- El papel esencial de Cks1 en la mitosis implica la regulación de la transcripción de Cdc20.
- Cks1 probablemente facilita la transcripción mediante el reclutamiento del proteosoma y / o la disociación de la cinasa Cdc28 del promotor CDC20.
- Esta acción remodela complejos transcripcionales o cromatina en el gen CDC20, promoviendo la división celular.
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