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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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Chromatin Structure Regulates pre-mRNA Processing02:41

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Una vista panorámica del procesamiento del ARN no codificante de la levadura.

Wen Tao Peng1, Mark D Robinson, Sanie Mnaimneh

  • 1Banting and Best Department of Medical Research, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada.

Cell
|July 3, 2003
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores descubrieron muchas nuevas proteínas de levadura involucradas en la biogénesis de las ribonucleoproteínas (RNP). El análisis de perfiles de ARN no codificantes reveló proteínas no caracterizadas cruciales para el procesamiento de ARN, ampliando nuestra comprensión de la síntesis de RNP.

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Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética de la levadura Genética de la levadura
  • La Proteómica es la Proteómica.

Sus antecedentes:

  • Las ribonucleoproteínas (RNP) son máquinas moleculares esenciales involucradas en numerosos procesos celulares.
  • El complemento completo de proteínas involucradas en la biogénesis de RNP, particularmente en la levadura, sigue siendo incompletamente caracterizado.
  • Los datos de genómica y proteómica funcionales ofrecen vías potenciales para identificar nuevos componentes.

Objetivo del estudio:

  • Identificar nuevas proteínas de levadura involucradas en la biogénesis de las ribonucleoproteínas (RNP).
  • Evaluar globalmente las funciones de las proteínas de levadura no caracterizadas en el procesamiento de ARN no codificante.

Principales métodos:

  • Utilizó el análisis predictivo de los datos de genómica funcional y proteómica de levaduras disponibles públicamente.
  • Empleó un microarray para monitorear la abundancia de ARN no codificante y el procesamiento a través de 468 cepas mutantes de levaduras.
  • Se llevaron a cabo más experimentos para verificar el papel de los factores identificados en la biogénesis del ARN no codificante.

Principales resultados:

  • El análisis predictivo sugirió que un mayor número de proteínas están involucradas en la biogénesis de RNP de lo que se conoce actualmente.
  • El análisis de 468 cepas de levadura reveló perfiles de ARN no codificantes aberrantes en mutantes de genes no caracterizados.
  • Se validaron experimentalmente diez factores involucrados en la biogénesis del ARN no codificante, incluidas las proteínas que afectan el procesamiento pre-ARNr, la función del exosoma nuclear y la acumulación de ARN sno.

Conclusiones:

  • Un número significativo de proteínas de levadura actualmente no caracterizadas juegan un papel crítico en la biogénesis del ARN no codificante.
  • Este estudio proporciona una visión global del procesamiento de ARN no codificante de la levadura e identifica nuevos factores de biogénesis de RNP.
  • Los hallazgos amplían el panorama conocido de proteínas involucradas en la síntesis y procesamiento de RNP en levaduras.