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Factores que determinan la resistencia a las proteínas de las monocapas autoensambladas de oligoéter: hidrofilicidad
Sascha Herrwerth1, Wolfgang Eck, Sven Reinhardt
1Angewandte Physikalische Chemie, Universität Heidelberg, INF 253, D 69120 Heidelberg, Germany.
Journal of the American Chemical Society
|August 2, 2003
Resumen
Las monocapas autoensambladas de oligoéter (SAM) resisten la adsorción de proteínas cuando tienen un interior y un exterior hidrófilos. La penetración de agua en el interior del SAM es crucial para lograr la resistencia a las proteínas en estas películas orgánicas "inertes".
Área de la Ciencia:
- Ciencias de la superficie Ciencias de la superficie.
- Ciencia de los materiales ciencia de los materiales.
- Los biomateriales son biomateriales.
Sus antecedentes:
- Las monocapas autoensambladas (SAM) son cruciales para la modificación de la superficie.
- Comprender la resistencia a las proteínas es vital para el desarrollo de biomateriales.
- Los SAMs Oligoether son prometedores para la creación de superficies inertes.
Objetivo del estudio:
- Investigar sistemáticamente los factores estructurales que influyen en la resistencia a las proteínas en SAMs de oligoéter.
- Para aclarar la relación entre la composición, estructura y adsorción de proteínas de SAM.
- Identificar parámetros clave para el diseño de superficies efectivas resistentes a las proteínas.
Principales métodos:
- Síntesis de alcanetiolos terminados por oligoéter con diferentes longitudes de cadena y terminaciones.
- Caracterización de SAMs usando XPS, mediciones de ángulo de contacto y espectroscopia IRRAS.
- Cuantificación de la adsorción de proteínas a través de la elipseometría.
Principales resultados:
- Los interiores hidrofóbicos (por ejemplo, oligo (((propilenglicol)) eliminan la resistencia de las proteínas.
- Los ángulos de contacto de agua altos (>70° en oro, >65° en plata) reducen la resistencia de las proteínas.
- La compresión lateral puede disminuir la resistencia a las proteínas en las SAMs de oligo (etilenglicol).
Conclusiones:
- Tanto la hidrofilicidad interna como la terminal son esenciales para la resistencia a las proteínas.
- La penetración de moléculas de agua en el interior del SAM es un requisito previo para la resistencia de las proteínas.
- El diseño de películas orgánicas "inertes" requiere un control cuidadoso de la hidrofilicidad y la estructura de SAM.
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