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Discriminación de secuencias por isoformas alternadamente empalmadas de un dominio de dedo de zinc de unión al ADN
1Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
Science (New York, N.Y.)
|September 25, 1992
Resumen
Dos formas del factor de transcripción del corión de Drosophila CF2 (CF2) tienen diferentes preferencias de unión al ADN debido a un dedo de zinc adicional. Esta diferencia permite que CF2 regule potencialmente conjuntos de genes distintos durante el desarrollo.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
Sus antecedentes:
- Existen dos isoformas principales del factor de transcripción del corión de Drosophila CF2.
- Estas isoformas se diferencian por un dedo de zinc adicional en el dominio de unión al ADN.
Objetivo del estudio:
- Para determinar los sitios preferidos de unión al ADN para cada isoforma CF2.
- Comprender cómo el empalme alternativo y las variaciones del dedo de zinc afectan la especificidad de unión al ADN.
Principales métodos:
- Determinación del sitio de unión al ADN para las isoformas CF2.
- Análisis de patrones de reconocimiento de secuencias.
Principales resultados:
- La isoforma con el dedo de zinc adicional se une preferentemente a sitios con una secuencia TAT duplicada.
- La especificidad de enlace está modulada por interacciones modulares entre dedos de zinc y trinucleótidos.
- Se sugirieron reglas para el reconocimiento del sitio de ADN rico en AT por las proteínas del dedo de zinc.
Conclusiones:
- El empalme alternativo y las interacciones modulares del dedo de zinc permiten una especificidad de unión alterada para CF2.
- Las isoformas CF2 pueden reconocer un espectro más amplio de sitios de ADN.
- CF2 puede regular conjuntos distintos de genes diana durante el desarrollo de Drosophila.
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