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Pinocytosis

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Cells use energy-requiring bulk transport mechanisms to transfer large particles, or large amounts of small particles, into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.

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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

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Encapsulación del huésped en una cápsula molecular soluble en agua basada en interacciones iónicas.

Francesca Corbellini¹, Luigi Di Costanzo, Mercedes Crego-Calama

¹Laboratory of Supramolecular Chemistry and Technology, Mesa+ Research Institute, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.

Journal of the American Chemical Society

|August 14, 2003

Resumen

Los investigadores desarrollaron una cápsula molecular soluble en agua utilizando interacciones iónicas. Este conjunto supramolecular exhibe una fuerte unión en el agua, lo que permite aplicaciones de reconocimiento molecular en entornos acuosos.

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Área de la Ciencia:

Química supramolecular de las moléculas.
Ciencia de los materiales Ciencia de los materiales.

Sus antecedentes:

El desarrollo de cápsulas moleculares solubles en agua es crucial para aplicaciones biológicas y químicas.
Las interacciones iónicas ofrecen una ruta prometedora para la construcción de ensamblajes supramoleculares robustos.

Objetivo del estudio:

Para sintetizar y caracterizar una nueva cápsula molecular soluble en agua.
Para investigar las propiedades de unión y las aplicaciones potenciales de este conjunto en medios acuosos.

Principales métodos:

Síntesis de la cápsula molecular.
Caracterización mediante espectroscopia de resonancia magnética nuclear de protones (1H NMR).
Espectrometría de masas de ionización por electrospray (ESI-MS) para la confirmación estructural.
Calorimetría de titulación isotérmica (ITC) para determinar la afinidad de unión.

Principales resultados:

Síntesis exitosa de una cápsula molecular soluble en agua.
Se ha demostrado una fuerte asociación en el agua con una constante de asociación (Ka) de 3.3 x 10^4 M^-1.
Confirmación de la estabilidad y solubilidad del conjunto en solución acuosa.

Conclusiones:

La cápsula molecular desarrollada es estable y soluble en agua.
La fuerte afinidad de unión del sistema lo hace adecuado para el reconocimiento molecular en agua pura.
Abre caminos para aplicaciones en sistemas de detección y suministro de fase acuosa.