Videos de Conceptos Relacionados
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:34Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
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Updated: Jun 21, 2026
12:02
Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
La proliferación celular sin la ciclina E-CDK2
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Cell
|August 28, 2003
Resumen
Los ratones pueden desarrollarse sin las ciclinas E1 y E2, pero estas proteínas son cruciales para la progresión del ciclo celular. Específicamente, las ciclinas E1 y E2 son esenciales para la endoreplicación y la quiescencia de salida en las células del ratón.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- Genética molecular genética molecular.
Sus antecedentes:
- Las ciclinas son reguladores clave del ciclo celular.
- Se sabe que las proteínas ciclina E (E1 y E2) juegan un papel en la proliferación celular.
- La necesidad específica de las ciclinas E1 y E2 en el desarrollo embrionario de mamíferos y la salida del ciclo celular no se ha aclarado completamente.
Objetivo del estudio:
- Investigar el papel de las ciclinas E1 y E2 en el desarrollo embrionario del ratón.
- Para determinar la función de las ciclinas E1 y E2 en la proliferación de los fibroblastos.
- Evaluar el requisito de las ciclinas E1 y E2 para la endoreplicación y la salida de la quiescencia.
Principales métodos:
- Generación de ratones que carecen tanto de los genes de la ciclina E1 como de la ciclina E2 (doble nocaut).
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08:33Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
12:26Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
Principales resultados:
- Los ratones que carecen de ciclinas E1 y E2 pueden completar el desarrollo embrionario.
- Los fibroblastos de los embriones de doble knockout muestran una proliferación normal in vitro.
- Las ciclinas E1 y E2 son indispensables para la endoreplicación y la transición de la quiescencia al ciclo celular activo.
Conclusiones:
- Las ciclinas E1 y E2 no son esenciales para el desarrollo embrionario o la proliferación de fibroblastos básicos.
- Estas ciclinas específicas juegan un papel crítico en los procesos especializados del ciclo celular, a saber, la endoreplicación y la salida de la quiescencia.