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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
Published on: January 27, 2012
La oscilación de la MPF se acompaña de una asociación periódica entre cdc25 y cdc2-ciclina B
Cell
|January 24, 1992
Resumen
Los ovocitos de Xenopus contienen p72, un pariente del cdc25 que activa el cdc2. p72 se asocia con cdc2-ciclina B durante la fase M, lo que sugiere que el control del ciclo celular implica interacciones con las proteínas, no solo la regulación génica.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Biología del desarrollo Biología del desarrollo.
Sus antecedentes:
- El inicio de la mitosis requiere la activación de la proteína quinasa cdc2.
- La activación del factor promotor de la maduración depende de la desfosforilación de la tirosina cdc2.
- cdc25 es la tirosina fosfatasa específica que activa el cdc2.
Objetivo del estudio:
- Para investigar el papel de un pariente de cdc25, p72, en óvulos y embriones de Xenopus.
- Para determinar si p72 interactúa con cdc2-ciclina B durante el ciclo celular.
- Explorar los mecanismos de control mitótico más allá de la regulación transcripcional.
Principales métodos:
- Identificación de la p72 en los ovocitos de Xenopus.
- Análisis de la abundancia de p72 durante el ciclo celular en embriones de Xenopus.
- Evaluación de la asociación de p72 con la cdc2-ciclina B.
- Análisis de la actividad catalítica de la quinasa asociada a la fase M.
Principales resultados:
- Los ovocitos de Xenopus poseen un relativo de cdc25, p72.
- La abundancia de p72 no oscila durante el ciclo celular en los embriones de Xenopus.
- p72 exhibe una asociación dependiente del ciclo celular con cdc2-ciclina B, con un pico en la fase M.
- La quinasa de fase M asociada con p72 es catalíticamente activa.
Conclusiones:
- El control mitótico puede implicar una asociación física periódica entre cdc25 y el complejo ciclina B-cdc2.
- El control mitótico puede ser influenciado por mecanismos distintos de la regulación transcripcional del gen cdc25.
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