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Efectos de la capilaridad en la cinética de la cristalización: insulina.
Ilya Reviakine1, Dimitra K Georgiou, Peter G Vekilov
1Department of Chemical Engineering, University of Houston, Houston, Texas 77204-4004, USA.
Journal of the American Chemical Society
|September 18, 2003
Resumen
La capilaridad gobierna la generación de la capa cristalina, con la longitud capilar 2D (L ((c)) que influye en las tasas de crecimiento de la capa. Este estudio confirma la densidad de pasos de crecimiento de las escalas L (c) en la cristalización de insulina porcina, con pasos más largos que crecen independientemente de la longitud.
Área de la Ciencia:
- Ciencia de la cristalización Ciencia de la cristalización.
- Física de la superficie Física de las superficies
- Ciencia de los materiales Ciencia de los materiales.
Sus antecedentes:
- La capilaridad es crucial para el crecimiento de cristales por capas, influyendo en la generación de nuevas capas.
- Los modelos teóricos vinculan la tensión de la línea de borde de la capa y la longitud capilar 2D (L) a la generación de capas y las tasas de crecimiento.
Objetivo del estudio:
- Investigar la correlación entre la longitud capilar 2D (L) y la tasa de generación de capas durante el crecimiento del cristal.
- Para validar experimentalmente las predicciones teóricas con respecto al papel de L (c) en la cinética de crecimiento de cristales.
Principales métodos:
- Se empleó microscopía de fuerza atómica (TM-AFM) para estudiar el crecimiento de los cristales.
- Se investigó la cristalización de la insulina porcina romboédrica (R3) debido a su adecuada cinética de crecimiento en pasos lineales.
Principales resultados:
- Se identificó la longitud capilar 2D (L) como el factor de escala primario para la densidad del paso de crecimiento.
- Pasos más largos que L (c) exhibieron tasas de crecimiento dependientes únicamente de la sobresaturación, independientemente de su longitud.
- Se midieron coeficientes cinéticos, comparables a los de los sistemas inorgánicos, que indican una alta sobresaturación en la cristalización de proteínas.
Conclusiones:
- La evidencia experimental apoya el papel teórico de L (c) para escalar la densidad del paso de crecimiento.
- Se observaron y explicaron las desviaciones de la teoría en las tasas de crecimiento escalonado a alta supersaturación.
- El estudio proporciona información sobre los mecanismos fundamentales que rigen el crecimiento de los cristales de proteínas.
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