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La fosforilación del factor de transcripción p62TCF por la MAP quinasa estimula la formación de complejos ternarios
H Gille1, A D Sharrocks, P E Shaw
1Max-Planck-Institut für Immunbiologie, Spemann Laboratories, Freiburg, Germany.
Nature
|July 30, 1992
Resumen
La proteína activada por mitógeno (MAP) quinasa fosforila el factor de transcripción p62TCF, mejorando su unión al promotor del gen c-FOS. Esta fosforilación es crucial para la expresión génica c-FOS inducida por el factor de crecimiento.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La señalización celular de las células.
- Los oncogenes son oncogenes.
Sus antecedentes:
- La transcripción del proto-oncogén c-FOS es rápidamente inducida por factores de crecimiento.
- Un complejo ternario de p67SRF y p62TCF se une al elemento de respuesta sérica, crucial para la inducción de c-FOS.
- La proteína quinasa activada por mitógenos (MAP) es activada por mitógenos y transmite señales proliferativas.
Objetivo del estudio:
- Para investigar el papel de la MAP quinasa en la regulación de la transcripción c-FOS.
- Para determinar si la MAP quinasa influye directamente en la formación del complejo ternario en el elemento de respuesta sérica c-FOS.
Principales métodos:
- Ensayos de fosforilación in vitro utilizando MAP quinasa y p62TCF.
- Estimulación de las células suizas 3T3 con el factor de crecimiento epidérmico (EGF) e insulina.
- Análisis de la expresión génica c-FOS y la formación del complejo ternario.
Principales resultados:
- Fosforilados de la quinasa MAP p62TCF in vitro, que mejoran la formación del complejo ternario.
- El tratamiento con el FEAG activa la MAP quinasa, lo que lleva a la expresión de c-FOS y a la formación activa del complejo ternario.
- El tratamiento con insulina, que no activa la MAP quinasa, no induce la transcripción c-FOS ni mejora la formación del complejo ternario.
Conclusiones:
- La fosforilación mediada por MAP quinasa de p62TCF es un paso clave en la expresión del proto-oncogén c-FOS inducido por el factor de crecimiento.
- Este estudio vincula las vías de señalización de la MAP quinasa con la regulación de la transcripción c-FOS.
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