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La aminopeptidasa N es un receptor principal para el coronavirus entero-patógeno TGEVV
B Delmas1, J Gelfi, R L'Haridon
1Unité de Virologie et Immunologie Moléculaires, INRA, Jouy-en-josas, France.
Nature
|June 4, 1992
Resumen
El virus de la gastroenteritis transmisible (TGEV) utiliza la aminopeptidasa N como su receptor para infectar a los cerdos. Este hallazgo identifica un determinante molecular clave para la entrada del TGEV en las células huésped.
Área de la Ciencia:
- Virología Veterinaria Virología Veterinaria
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
Sus antecedentes:
- Los coronavirus exhiben un rango restringido de huéspedes y tropismo tisular.
- El virus de la gastroenteritis transmisible (TGEV) causa diarrea fatal en lechones al infectar los enterocitos del intestino delgado.
Objetivo del estudio:
- Para identificar la molécula de superficie específica que media en la unión y entrada de TGEV.
- Para dilucidar los mecanismos moleculares de la interacción de la célula huésped con el TGEV.
Principales métodos:
- Selección de anticuerpos monoclonales que bloquean la infección por TGEV en líneas celulares porcinas.
- Identificación de la proteína diana mediante el reconocimiento de anticuerpos y la secuenciación amino-terminal.
- Purificación de la proteína diana y evaluación de la unión viral.
- Expresión recombinante de la proteína identificada en las células no permisivas.
Principales resultados:
- Se identificó una proteína de membrana de borde de cepillo de 150K como aminopeptidasa N.
- La aminopeptidasa N fue confirmada como el receptor del TGEV a través de ensayos específicos de unión al virión.
- La expresión de N de aminopeptidasa recombinante hizo que las células no permisivas fueran susceptibles a la infección por TGEV.
Conclusiones:
- La aminopeptidasa N es el receptor primario para el virus de la gastroenteritis transmisible (TGEV) en los enterocitos porcinos.
- La comprensión de esta interacción es crucial para el desarrollo de estrategias de control de TGEV.
- Este estudio aclara un determinante clave del huésped viral para la patogénesis del coronavirus.
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