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Dominios de expresión anidados de cuatro genes homeobox en el desarrollo del cerebro rostral en desarrollo
A Simeone1, D Acampora, M Gulisano
1International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Nature
|August 20, 1992
Resumen
Al investigar el desarrollo de la cabeza en ratones, los investigadores identificaron cuatro genes clave: Emx1, Emx2, Otx1 y Otx2. Sus patrones de expresión revelan dominios anidados cruciales para la regionalización temprana del cerebro.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La neurociencia es la neurociencia.
- Genética La genética.
Sus antecedentes:
- Comprender el desarrollo de la cabeza de los vertebrados es limitado, especialmente en comparación con el desarrollo del tronco.
- Los genes de Drosophila como los espirales vacíos y el ortodenticulo son cruciales para el desarrollo de la cabeza.
- Los genes Emx1 y Emx2 del ratón, relacionados con las espirales vacías, se expresan en el cerebro anterior en desarrollo.
Objetivo del estudio:
- Para identificar y caracterizar los genes de ratón relacionados con Drosophila orthodenticle.
- Para comparar los dominios de expresión de Emx1, Emx2, Otx1 y Otx2 en el cerebro del ratón en desarrollo.
- Para aclarar el papel de estos genes en la especificación regional del cerebro anterior.
Principales métodos:
- Clonado y caracterización de los genes Otx1 y Otx2 en ratones.
- Análisis comparativo de los patrones de expresión génica utilizando embriones de ratón al día 10 después del coito.
- Mapeo de los dominios de expresión de Emx1, Emx2, Otx1 y Otx2 en el desarrollo del cerebro rostral.
Principales resultados:
- Otx2 se expresa ampliamente en el telencéfalo, el diencefalo y el mesencefalo.
- La expresión Otx1 está anidada dentro del dominio Otx2.
- Emx2 y Emx1 muestran dominios de expresión progresivamente restringidos dentro del telencéfalo dorsal y el diencefalo.
Conclusiones:
- Los cuatro genes (Emx1, Emx2, Otx1, Otx2) exhiben patrones de expresión anidados en el cerebro en desarrollo del ratón.
- Estos dominios anidados se establecen temprano en el desarrollo, coincidiendo con la especificación regional de las principales áreas cerebrales.
- Los hallazgos proporcionan información sobre el control genético de los patrones del cerebro anterior en los vertebrados.
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