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Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique
Published on: December 21, 2010
Regulación del desarrollo de las corrientes sinápticas mediadas por el receptor NMDA en una sinapsis central
1Department of Physiology, University of California School of Medicine, San Francisco 94143.
Nature
|June 25, 1992
Resumen
La plasticidad cerebral en los primeros años de vida involucra a los receptores de N-metil-D-aspartato (NMDA). Este estudio muestra una función alterada del receptor NMDA durante el desarrollo, lo que sugiere que los cambios moleculares controlan la plasticidad sináptica en cerebros jóvenes.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología del desarrollo Biología del desarrollo.
- La plasticidad sináptica.
Sus antecedentes:
- El sistema nervioso central exhibe una plasticidad significativa durante el desarrollo temprano.
- La transmisión sináptica excitatoria rápida está mediada por el N-metil-D-aspartato (NMDA) y los receptores no NMDA.
- Estudios anteriores sugirieron mejoras transitorias en la función del receptor NMDA a principios de la vida, pero faltaba evidencia funcional directa debido a desafíos técnicos.
Objetivo del estudio:
- Para investigar los cambios en el desarrollo de la transmisión sináptica mediada por el receptor NMDA.
- Para determinar si la cinética de las corrientes de los receptores NMDA cambia durante el desarrollo temprano del cerebro.
- Explorar las bases moleculares de la plasticidad sináptica en el sistema nervioso central en desarrollo.
Principales métodos:
- Registros electrofisiológicos de corrientes postsinápticas excitatorias evocadas y en miniatura (e.p.s.c.) en el colículo superior.
- Comparación de la duración y amplitud de la e.p.s.c mediada por el receptor NMDA entre las primeras etapas del desarrollo y los animales más viejos.
- Análisis de parches de membrana extirpados para evaluar la cinética del canal del receptor NMDA.
Principales resultados:
- La duración de la e.p.s.c. evocada mediada por el receptor NMDA fue significativamente más larga en las primeras etapas del desarrollo en comparación con los animales más viejos.
- La amplitud de las e.p.s.c. mediadas por receptores NMDA en miniatura se mantuvo sin cambios a lo largo del desarrollo.
- Las propiedades cinéticas de los receptores NMDA en los parches extirpados reflejaron el curso de tiempo prolongado observado en los e.p.s.c. evocados, lo que indica una cinética lenta del canal.
Conclusiones:
- El curso temporal de las corrientes sinápticas mediadas por el receptor NMDA en el colículo superior en desarrollo está determinado principalmente por las propiedades lentas del canal NMDA.
- Estos hallazgos demuestran que las propiedades moleculares de los receptores NMDA están reguladas en el desarrollo.
- La regulación del desarrollo de las propiedades del receptor NMDA probablemente juega un papel crucial en el control de la plasticidad sináptica durante la vida temprana.
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