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La S-nitrosilación del NSF controla el tráfico de membranas
Thomas H Söllner1, Sonia Sequeira
1Memorial Sloan-Kettering Cancer Center, Cellular Biochemistry & Biophysics Program, New York, NY 10021, USA.
Cell
|October 22, 2003
Resumen
El óxido nítrico regula la exocitosis mediante la S-nitrocilación del NSF, una ATPasa. Esta inhibición afecta a la liberación de los cuerpos de Weibel-Palade, cruciales para la regulación del tono vascular en las células endoteliales.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología molecular La biología molecular.
- Fisiología Fisiología Fisiología.
Sus antecedentes:
- El óxido nítrico (NO) es una molécula de señalización clave que afecta a varios procesos biológicos.
- La exocitosis regulada, el proceso de liberación del contenido celular, está influenciada por el NO, pero sus objetivos moleculares no se comprenden completamente.
- Los cuerpos de Weibel-Palade son gránulos secretores especializados en las células endoteliales vitales para la homeostasis vascular.
Objetivo del estudio:
- Para identificar los objetivos moleculares del óxido nítrico en la regulación de la exocitosis.
- Investigar el mecanismo por el cual el óxido nítrico afecta la liberación de los cuerpos de Weibel-Palade de las células endoteliales aórticas.
Principales métodos:
- El estudio se centró en las células endoteliales aórticas.
- Los investigadores investigaron la S-nitrosilación de NSF (factor sensible a la N-etilmaleimida), una ATPasa involucrada en la fusión de la membrana.
- Se evaluó el efecto de la S-nitrosilación de NSF en la exocitosis de los cuerpos de Weibel-Palade.
Principales resultados:
- El óxido nítrico induce la S-nitrosilación de NSF en las células endoteliales aórticas.
- La S-nitrosilación de NSF inhibe su actividad ATPasa, crucial para la fusión de la membrana.
- Esta inhibición conduce a la supresión de la exocitosis del cuerpo de Weibel-Palade.
Conclusiones:
- NSF es un objetivo molecular del óxido nítrico en las células endoteliales.
- La S-nitrosilación de NSF por el óxido nítrico es un mecanismo para inhibir la exocitosis de los cuerpos de Weibel-Palade.
- Este hallazgo proporciona información sobre la regulación del tono vascular por medio de la señalización del óxido nítrico.
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