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Estructura del portador mitocondrial de ADP/ATP en complejo con el carboxyatractyloside
Eva Pebay-Peyroula1, Cécile Dahout-Gonzalez, Richard Kahn
1Institut de Biologie Structurale, UMR 5075 CEA-CNRS-Université Joseph Fourier, 41 rue Jules Horowitz, F-38027, Grenoble cedex 1, France. pebay@ibs.fr
Nature
|November 7, 2003
Resumen
La proteína portadora del ADP/ATP es la proteína ADP/ATP.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Estructural Biología estructural.
- Fisiología mitocondrial Fisiología mitocondrial
Sus antecedentes:
- El trifosfato de adenosina (ATP) es la fuente de energía primaria de la célula, generada en las mitocondrias.
- La exportación de ATP mitocondrial y la importación de ADP son cruciales para el metabolismo energético celular.
- La proteína portadora de ADP/ATP facilita este intercambio esencial a través de la membrana mitocondrial interna.
Objetivo del estudio:
- Para determinar la estructura de alta resolución del portador de ADP/ATP bovino.
- Para dilucidar el mecanismo molecular del transporte de ADP/ATP.
Principales métodos:
- Se empleó cristalografía de rayos X para resolver la estructura del portador bovino de ADP/ATP.
- La estructura se determinó con una resolución de 2,2 Å en complejo con el inhibidor carboxyatractyloside.
Principales resultados:
- La estructura revela un dominio transmembrana compacto compuesto por seis hélices alfa.
- Una depresión profunda en la superficie de la proteína conduce a una cavidad que contiene un hexapéptido conservado (RRRMMM).
- El inhibidor carboxyatractyloside estaba unido dentro de esta cavidad.
Conclusiones:
- La estructura sugiere que los sustratos se unen en la parte inferior de la cavidad.
- Se propone que el transporte ocurra a través de una transición conformacional de un estado de "foso" a un estado de "canal".
- Esto proporciona una base estructural para comprender el intercambio de nucleótidos mitocondriales.
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