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El ubicuo transportador de glucosa GLUT-1 es un receptor para el HTLV
Nicolas Manel1, Felix J Kim, Sandrina Kinet
1Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535/IFR 122, F-34293 Montpellier Cedex 5, France.
Cell
|November 19, 2003
Resumen
El virus de la leucemia de células T humanas (HTLV) utiliza el transportador de glucosa GLUT-1 como su receptor. Esta interacción interrumpe el transporte de glucosa, lo que contribuye potencialmente a las enfermedades asociadas con el HTLV.
Área de la Ciencia:
- Virología Virología.
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- El virus de la leucemia de células T humanas (HTLV) está relacionado con la leucemia y los trastornos neurológicos.
- El receptor específico para el HTLV y su papel en la patogénesis de la enfermedad siguen sin estar claros.
- Las envolturas de HTLV están implicadas en los efectos del virus, pero sus interacciones precisas no se comprenden completamente.
Objetivo del estudio:
- Para identificar el receptor celular para el HTLV.
- Esclarecer el mecanismo por el cual el HTLV envuelve, media la infección y causa la enfermedad.
- Para investigar el papel del transporte de glucosa en la patogénesis del HTLV.
Principales métodos:
- Investigó la interacción entre las glicoproteínas de envoltura HTLV-1 y HTLV-2 y el transportador de glucosa GLUT-1.
- Utilizó citocalasina B para inhibir el transporte de glucosa y siRNAs para bloquear la expresión de GLUT-1.
- Evaluación de los niveles de infección por HTLV tras la manipulación de la expresión de GLUT-1 y el transporte de glucosa.
Principales resultados:
- Las glicoproteínas de la envoltura de HTLV-1 y HTLV-2 se unen a GLUT-1, inhibiendo el transporte de glucosa.
- La inhibición del transporte de glucosa o la expresión de GLUT-1 bloquea la unión de la envolvente HTLV y la infección.
- La expresión ectópica de GLUT-1 restauró la infección por HTLV, mientras que GLUT-3 no lo hizo.
Conclusiones:
- GLUT-1 se identifica como el receptor celular para el HTLV.
- Las glicoproteínas de la envoltura HTLV interactúan con GLUT-1, afectando el metabolismo de la glucosa.
- Es probable que estas perturbaciones metabólicas contribuyan a las patologías asociadas con el HTLV.
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