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In vivo and in vitro Studies of Adaptor-clathrin Interaction
Published on: January 26, 2011
Una vía para la asociación de receptores, adaptadores y actina durante la internalización endocítica
Marko Kaksonen1, Yidi Sun, David G Drubin
1Department of Molecular and Cell Biology, 16 Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA.
Cell
|November 19, 2003
Resumen
La endocitosis de la levadura en ciernes implica cambios en las proteínas en los parches corticales, que se correlacionan con la motilidad. La polimerización de la actina es crucial para la internalización y el desmontaje de parches, con complejos que actúan como sitios de nucleación.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología molecular y celular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La internalización endocítica en la levadura en ciernes implica proteínas localizadas en parches corticales.
- Estos parches exhiben diferentes composiciones de proteínas y patrones de motilidad.
Objetivo del estudio:
- Para definir la vía endocítica temprana en la levadura en ciernes.
- Para correlacionar los cambios en la composición de la proteína del parche cortical con la motilidad del parche.
- Investigar el papel de la polimerización de actina y proteínas específicas en la endocitosis.
Principales métodos:
- Microscopía de fluorescencia multicolor en tiempo real.
- Algoritmos de seguimiento de partículas.
- Recuperación de la fluorescencia después del foto blanqueamiento (FRAP).
Principales resultados:
- Una secuencia invariante de cambios en la composición de las proteínas del parche cortical se correlaciona con la motilidad.
- Tres activadores Arp2/3 muestran comportamientos distintos, lo que indica funciones especializadas.
- La polimerización de la actina se produce tarde, es esencial para la internalización y el desmontaje del parche.
- La pérdida de Sla2p detiene la motilidad de los parches y forma colas de cometa de actina.
- Los complejos endocíticos sirven como sitios de nucleación para la polimerización de actina.
Conclusiones:
- El estudio define una secuencia temporal de cambios en la composición de proteínas y la motilidad en la endocitosis de la levadura.
- La polimerización de la actina es un evento de etapa tardía crítico para la internalización y el desmontaje.
- Los complejos endocíticos se identifican como sitios de nucleación de actina.
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