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Biosíntesis de cristales de CaCO3 de morfología compleja utilizando un hongo y un actinomiceto
Debabrata Rautaray1, Absar Ahmad, Murali Sastry
1Materials Chemistry and Biochemical Sciences Division, National Chemical Laboratory, Pune-411 008, India.
Journal of the American Chemical Society
|December 4, 2003
Resumen
Microorganismos como los hongos y los actinomicetos pueden crear cristales de carbonato de calcio (CaCO3). Estos microbios producen proteínas específicas que controlan la forma y el tipo de cristal, ofreciendo un método de formación de cristales totalmente biogénico.
Área de la Ciencia:
- La biomineralización es la biomineralización.
- Microbiología Microbiología.
- Ciencia de los materiales Ciencia de los materiales.
Sus antecedentes:
- El carbonato de calcio (CaCO3) es un material ampliamente utilizado.
- La síntesis biogénica de CaCO3 ofrece nuevas rutas para la producción de cristales.
- La influencia microbiana en la formación de minerales es un área de investigación activa.
Objetivo del estudio:
- Para investigar la biosíntesis del carbonato de calcio (CaCO3) por microorganismos específicos.
- Analizar el papel de las proteínas secretadas por los microbios en el control de la morfología y cristalografía de los cristales de CaCO3.
- Para demostrar un enfoque completamente biogénico para la producción de cristales de CaCO3.
Principales métodos:
- El cultivo de Fusarium sp. se lleva a cabo (hongos) y el Rhodococcus sp. (actinomicético). también conocido como actinomycete.
- Análisis de productos cristalinos de CaCO3 utilizando microscopía electrónica de barrido (SEM).
- Identificación de proteínas secretadas por microbios involucradas en la biomineralización.
Principales resultados:
- Fusarium sp. es una especie de Fusarium. produjo cristales de calcita en forma de cruz.
- Rhodococcus sp. es una especie de Rhodococcus produjo vaterita, un polimorfo inestable de CaCO3.
- Se identificaron proteínas secretadas por microbios como factores clave para controlar la morfología y el tipo de cristal.
- Los microorganismos proporcionaron CO2, lo que permitió una síntesis biogénica completa.
Conclusiones:
- Los hongos y los actinomicetos pueden sintetizar biogénicamente CaCO3.
- Las proteínas microbianas secretadas son cruciales para controlar el polimorfismo y la morfología del CaCO3.
- Este método basado en microbios ofrece una ruta sostenible para producir estructuras cristalinas específicas de CaCO3.
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