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A Caenorhabditis elegans Model System for Amylopathy Study
Published on: May 17, 2013
El envolvimiento de cadáveres celulares mediado por el receptor de fosfatidilserina de C. elegans a través de CED-5 y
Xiaochen Wang1, Yi-Chun Wu, Valerie A Fadok
1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.
Science (New York, N.Y.)
|December 4, 2003
Resumen
La fosfatidilserina (PS) expuesta en las células apoptóticas actúa como una señal de comerme. El receptor PSR-1 de Caenorhabditis elegans reconoce el PS, mediando el envolvimiento del cadáver celular a través de la vía CED-2/5/10/12.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología molecular La biología molecular.
- Biología del desarrollo Biología del desarrollo.
Sus antecedentes:
- La exposición a la fosfatidilserina (PS) en las células apoptóticas señala la fagocitosis.
- El mecanismo de reconocimiento de PS por los fagocitos no se entiende completamente.
- Se ha propuesto un presunto receptor de fosfatidilserina (PSR) para mediar este reconocimiento.
Objetivo del estudio:
- Para investigar el papel del homólogo de Caenorhabditis elegans de PSR (PSR-1) en el envolvimiento de cadáveres celulares.
- Para determinar si PSR-1 reconoce la fosfatidilserina y media la fagocitosis.
Principales métodos:
- Estudió la función de psr-1 en C. elegans.
- Se realizaron ensayos de unión in vitro de la PSR-1.
- Se analizó la interacción de PSR-1 con la vía de señalización CED-2/5/10/12.
Principales resultados:
- La PSR-1 es esencial para el envolvimiento de cadáveres celulares en C. elegans.
- In vitro, PSR-1 se une preferentemente a la fosfatidilserina o a las células con PS expuestas.
- PSR-1 actúa aguas arriba en la vía de señalización CED-2/5/10/12, interactuando potencialmente con CED-5 y CED-12.
Conclusiones:
- La PSR-1 funciona como un receptor de fosfatidilserina en el C. elegans.
- La PSR-1 juega un papel crucial en el reconocimiento de la señal "cómete a mí" durante la fagocitosis.
- La PSR-1 es un componente aguas arriba de la conservada vía de envuellamiento de cadáveres celulares CED-2/5/10/12.
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