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Interacción del snRNA U6 con una secuencia requerida para la función del ARN SL del nematodo en el trans-splicing
G J Hannon1, P A Maroney, Y T Yu
1Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
Science (New York, N.Y.)
|December 11, 1992
Resumen
Los ARN del líder empalmado de nematodos (SL) impulsan el trans-esplicado a través de su dominio similar al snRNA, no del exón. Los elementos de secuencia específicos dentro de este dominio son cruciales para la actividad del ARN SL en las reacciones de empalme.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- ARN Biología Biología ARN
- Genética La genética.
Sus antecedentes:
- Los ARN del líder empalmado de nematodos (SL) son esenciales para el trans-esplicado.
- Los ARN SL consisten en un exón y un pequeño ARN nuclear (ARN sn) similar al dominio.
Objetivo del estudio:
- Identificar los dominios funcionales y los elementos de secuencia de los ARN SL de nematodos requeridos para el trans-splicing.
- Para investigar el papel del dominio snRNA-como en la actividad de ARN SL.
Principales métodos:
- El análisis de interferencia de la modificación química se empleó para sondear las relaciones estructura-función del ARN SL.
- Se utilizaron ensayos de transsplicing in vitro para evaluar la actividad de los ARN SL modificados y los ARN híbridos.
Principales resultados:
- La participación del ARN SL en el trans-splicing está dictada por el dominio similar al snRNA, independiente del exón.
- Dos elementos de secuencia específicos dentro del dominio snRNA-como son esenciales para la actividad de ARN SL.
- Un ARN híbrido compuesto de un fragmento de U1 snRNA y estos elementos podrían participar en el trans-splicing.
Conclusiones:
- El dominio similar al snRNA de los ARN SL del nematodo contiene elementos críticos para el trans-splicing.
- Estos elementos son suficientes para conferir la actividad de trans-splicing.
- Un elemento puede funcionar a través del emparejamiento de bases con U6 snRNA, destacando su papel en la maquinaria de empalme.
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