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Updated: Jul 5, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
19:16

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

Determinación del estado de protonación del sitio activo de la beta-secretasa a partir de la simulación de dinámica

Hwangseo Park1, Sangyoub Lee

  • 1School of Chemistry and Molecular Engineering, and Center for Molecular Catalysis, Seoul National University, Seoul 151-747, South Korea. hwangseo@snu.ac.kr

Journal of the American Chemical Society
|December 25, 2003
PubMed
Resumen

Memapsin 2 (BACE), crucial en la enfermedad de Alzheimer, tiene estados ambiguos de protonación del ácido aspártico. Las simulaciones revelan que Asp228, no Asp32, actúa como el receptor de enlace de hidrógeno para los inhibidores, guiando el diseño de fármacos.

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