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Nox4 como el principal componente catalítico de una NAD(P) H oxidasa endotelial
Tetsuro Ago1, Takanari Kitazono, Hiroaki Ooboshi
1Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. agou@intmed2.med.kyushu-u.ac.jp
Circulation
|January 14, 2004
Resumen
Nox4 es la principal fuente de especies reactivas de oxígeno en las células endoteliales vasculares, contribuyendo a las enfermedades vasculares. Este estudio identifica a Nox4 como el componente primario de la oxidasa endotélica NAD(P) H.
Área de la Ciencia:
- Biología Vascular Biología Vascular
- La señalización celular de las células.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las especies reactivas de oxígeno (ROS) son moléculas de señalización críticas en las células vasculares.
- La NAD(P) H oxidasa es la fuente primaria de ROS en las células endoteliales vasculares.
- La estructura exacta y la activación de esta oxidasa siguen sin estar claras.
Objetivo del estudio:
- Investigar la identidad molecular y la actividad productora de superóxido de la NAD(P) H oxidasa endotelial.
- Determinar el papel de Nox4 en la producción de ROS endoteliales.
Principales métodos:
- Expresó Nox4 en células endoteliales y lo comparó con otras proteínas Nox.
- Se midió la producción de superóxido utilizando resonancia de espín de electrones y quimioluminiscencia.
- Nox4 regulado a la baja utilizando oligonucleótidos antisense para evaluar sus efectos in vivo e in vitro.
Principales resultados:
- Nox4 fue altamente expresado en las células endoteliales, superando a otras proteínas Nox como gp91phox/Nox2.
- Las membranas endoteliales exhibieron una producción significativa de superóxido dependiente de NAD(P) H.
- La producción de superóxido se redujo por la regulación a la baja de Nox4, lo que confirma su papel.
Conclusiones:
- Nox4 es probablemente el principal componente catalítico de la NAD(P) H oxidasa endotelial.
- Nox4 juega un papel clave en la generación de ROS en las células endoteliales vasculares.
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