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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
Published on: October 7, 2011
Hipovirulencia del hongo de la plaga de la castaña conferida por un cDNA viral infeccioso
1Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Nutley, NJ 07110.
Science (New York, N.Y.)
|August 7, 1992
Resumen
Ciertos virus reducen los efectos dañinos del hongo Cryphonectria parasitica. Estas cepas hipovirulentas pueden ser diseñadas, ofreciendo una nueva estrategia de biocontrol contra este patógeno vegetal.
Área de la Ciencia:
- La micología de la micología.
- Patología Vegetal Patología vegetal.
- Virología Virología.
Sus antecedentes:
- La plaga del castaño, causada por Cryphonectria parasitica, devasta las poblaciones de castaño.
- Las cepas hipovirulentas de C. parasitica, a menudo infectadas con ARN viral de doble cadena (ARNds), exhiben una patogenicidad reducida.
- Estas cepas hipovirulentas pueden convertir las cepas virulentas a un estado menos dañino a través de la anastomosis.
Objetivo del estudio:
- Confirmar el dsRNA viral como el agente causante de la hipovirulencia en C. parasitica.
- Demostrar la viabilidad de la ingeniería de cepas de hongos hipovirulentos para el control biológico.
Principales métodos:
- Transformación de la virulenta C. parasitica con una copia de ADNc del ARN viral asociado a la hipovirulencia.
- Resurrección del dsRNA citoplasmático desde el cDNA integrado.
- Prueba de la capacidad del dsRNA resucitado para convertir cepas virulentas.
Principales resultados:
- Las cepas transformadas exhibieron el fenotipo completo de hipovirulencia.
- El dsRNA resucitado convirtió con éxito cepas virulentas compatibles en hipovirulencia.
- El dsRNA viral fue confirmado como el agente causal de la hipovirulencia.
Conclusiones:
- El dsRNA viral es definitivamente la causa de la hipovirulencia en Cryphonectria parasitica.
- La ingeniería de cepas fúngicas hipovirulentas es un enfoque viable de biocontrol para la peste del castaño.
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