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La secreción de factores angiogénicos y antiapoptóticos por las células estomales adiposas humanas
Jalees Rehman1, Dmitry Traktuev, Jingling Li
1Krannert Institute of Cardiology, Indiana Center for Vascular Biology & Medicine, Indianapolis, Ind 46202, USA.
Circulation
|March 3, 2004
Resumen
Las células estomales adiposas subcutáneas (CSA) son una fuente prometedora para la terapia de enfermedades cardiovasculares. Estas células mejoran el crecimiento y la supervivencia de los vasos sanguíneos, ofreciendo una nueva opción terapéutica para promover la angiogénesis.
Área de la Ciencia:
- La Medicina Regenerativa es una Medicina Regenerativa.
- Investigación Cardiovascular Investigación Cardiovascular Investigación Cardiovascular Investigación Cardiovascular Investigación Cardiovascular
- Terapia celular Terapia celular La terapia celular es la terapia que se utiliza para tratar el cáncer.
Sus antecedentes:
- El tratamiento de las enfermedades cardiovasculares está explorando la entrega de células autólogas para promover la angiogénesis.
- El número limitado de células puede obstaculizar la efectividad de las terapias actuales basadas en células.
- Las células estomales adiposas (ASC) de la grasa subcutánea ofrecen una nueva fuente celular accesible.
Objetivo del estudio:
- Investigar el potencial de las células estomales no adipocíticas de la grasa subcutánea humana como fuente de angiogénesis terapéutica.
- Para caracterizar el perfil de secreción del factor de crecimiento de ASCs.
- Evaluar los efectos angiogénicos y antiapoptóticos de las CSA in vitro e in vivo.
Principales métodos:
- Caracterización por aislamiento y citometría de flujo de células estomales adiposas subcutáneas humanas (CSA).
- Cuantificación de la secreción del factor de crecimiento (VEGF, HGF, TGF-β) por los ASC en condiciones normóxicas e hipóxicas.
- Evaluación de los efectos de los medios condicionados en la proliferación de células endoteliales y la apoptosis.
- Evaluación in vivo de ASC en un modelo de ratón de extremidad trasera isquémica.
Principales resultados:
- Las ASC secretan cantidades significativas de VEGF, HGF y TGF-β.
- La hipoxia y la transfección del gen VEGF aumentaron notablemente la secreción de VEGF de los ASC.
- Los medios condicionados de ASCs hipóxicos promovieron el crecimiento de las células endoteliales y redujeron la apoptosis.
- El tratamiento de la CSA humana mejoró significativamente la perfusión en ratones con extremidades traseras isquémicas.
Conclusiones:
- Las CSA subcutáneas poseen un potencial angiogénico y antiapoptótico debido a la secreción de factores de crecimiento proangiogénicos sinérgicos.
- Los ASC nativos o transducidos, regulados por la hipoxia, representan una nueva estrategia terapéutica para mejorar la angiogénesis.
- Estos hallazgos apoyan el uso de ASCs autólogas para la protección y regeneración cardiovascular.
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