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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
Published on: January 21, 2020
Regulación génica: antiguas secuencias diana de microARN en las plantas
Sandra K Floyd1, John L Bowman
1Section of Plant Biology, University of California, Davis, California 95616, USA.
Nature
|April 2, 2004
Resumen
Los microARN regulan la expresión génica en las plantas. Este estudio muestra un mecanismo específico de regulación de microARN en la familia de genes de la clase III homeodomain-leucina zipper (HD-Zip) se conserva en todas las plantas terrestres, que se remonta a más de 400 millones de años.
Área de la Ciencia:
- Biología vegetal Biología vegetal
- Genética molecular genética molecular.
- Biología evolutiva Biología evolutiva.
Sus antecedentes:
- Los microARN (miRNA) son pequeñas moléculas de ARN que regulan la expresión génica en los eucariotas.
- La familia de genes homeodomain-leucina zipper (HD-Zip) de clase III juega un papel crucial en el desarrollo de las plantas.
- Estudios previos indicaron la regulación por miRNA de los genes HD-Zip en plantas con flores.
Objetivo del estudio:
- Para investigar la conservación evolutiva de la regulación de miRNA dirigida a los genes HD-Zip en diversos linajes de plantas terrestres.
- Para determinar si el sitio de unión del miARN y el mecanismo de escisión se conservan en las plantas no florecientes.
Principales métodos:
- Análisis bioinformático de secuencias homólogas de varios grupos de plantas terrestres (briófitas, licópodos, helechos, plantas de semilla).
- Validación experimental de la escisión del ARN mensajero (ARNm) dentro del sitio de unión del microARN conservado.
Principales resultados:
- La secuencia objetivo de dos miRNAs específicos en los genes de Arabidopsis HD-Zip se conserva en todos los principales linajes de plantas terrestres.
- Los ARN mensajeros de genes HD-Zip homólogos se escinden en el sitio de unión de microARN conservado en representantes de cada grupo de plantas terrestres.
- Evidencia de una regulación génica mediada por miARN conservada tanto en plantas con y sin flores.
Conclusiones:
- La regulación genética mediada por microARN es un mecanismo antiguo en las plantas terrestres, que precede a la evolución de las plantas con flores.
- La regulación de los genes HD-Zip de clase III por microARN específicos se ha conservado durante más de 400 millones de años de evolución de las plantas.
- Esta vía reguladora conservada destaca la importancia fundamental de los microARN en el desarrollo y la evolución de las plantas.
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