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Función del factor desencadenante y DnaK en el plegamiento de proteínas multidominio: aumento del rendimiento a

Vishwas R Agashe¹, Suranjana Guha, Hung-Chun Chang

¹Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.

|April 16, 2004

Resumen

El factor desencadenante de las chaperonas bacterianas y el DnaK mejoran el rendimiento de plegamiento de proteínas pero retrasan el plegamiento. Existen diferencias en el acoplamiento de plegado traslacional entre las células bacterianas y las eucariotas.

Área de la Ciencia:

Biología molecular La biología molecular.
El plegamiento de las proteínas.
Síntesis de proteínas bacterianas.

Sus antecedentes:

El factor desencadenante y el DnaK son cruciales para prevenir el mal plegamiento de proteínas en E. coli.
El impacto preciso de estas chaperonas en los mecanismos de plegamiento de las proteínas de novo sigue sin estar claro.

Objetivo del estudio:

Investigar cómo el factor desencadenante y el DnaK influyen en el plegamiento de novo de proteínas multidominio durante la traducción.
Para comparar la eficiencia de plegado cotranslacional de proteínas bacterianas y eucariotas dentro del sistema E. coli.

Principales métodos:

Expresión de la beta-galactosidasa bacteriana y la luciferasa eucariota bajo condiciones de escasez de chaperona en E. coli.
Análisis in vivo e in vitro de la cinética de plegamiento de proteínas y rendimiento.
Investigación del reclutamiento de factores desencadenantes para la traducción de ribosomas.

Principales resultados:

Las proteínas multidominio se pliegan rápidamente pero de manera ineficiente a través de una vía predeterminada acoplada a la traducción.
El factor de activación y el DnaK mejoran el rendimiento de plegado, pero ralentizan significativamente el proceso de plegado.
El plegamiento de la luciferasa en E. coli sigue siendo ineficiente, a diferencia de los sistemas eucariotas, lo que indica incompatibilidad con el sistema de chaperón bacteriano.

Conclusiones:

Las chaperonas bacterianas modulan el plegamiento de las proteínas retrasando el proceso, lo que requiere un reclutamiento dinámico a los ribosomas.
Existen diferencias significativas en el acoplamiento de la traducción y el plegamiento entre los sistemas bacteriano y eucariótico.

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