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Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
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La fragmentación oxidativa de los hidroxi octadecadienoatos genera gamma-hidroxialquenales biológicamente activos
Mingjiang Sun1, Robert G Salomon
1Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44107-7078, USA.
Journal of the American Chemical Society
|May 6, 2004
Resumen
Los hidroxidienos, abundantes in vivo, pueden fragmentarse para formar gamma-hidroxialquenales citotóxicos cuando están presentes los hidroperoxidos. Esta vía es crucial para comprender la oxidación lipídica y el desarrollo de enfermedades.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Los lipidómicos son los lipídicos.
- El estrés oxidativo es el estrés oxidativo.
Sus antecedentes:
- La fragmentación de los ácidos grasos poliinsaturados (PUFA) genera aldehídos citotóxicos.
- Las fosfatidilcolinas gamma-hydroxyalkenal (PC) son biológicamente activas e implicadas en las enfermedades.
Objetivo del estudio:
- Para investigar la fragmentación oxidativa de los hidroxidienos, un producto común de oxidación de lípidos in vivo.
- Determinar el papel de los hidroxidienos como intermediarios en la generación de gamma-hidroxialquenales.
Principales métodos:
- Utilizó un estudio de modelo fisiomimético con pequeñas vesículas unilamellares.
- Fragmentación inducida por radicales libres promovida por la mieloperoxidasa empleada.
- Se analizaron los productos de fragmentación de los hidroxi- e hidroperoxidienos.
Principales resultados:
- Los hidroxidienos se fragmentan en gamma-hidroxialquenales en presencia de hidroperoxidos.
- Se observó la formación del éster HODA-PC del ácido 9-hidroxi-12-oxododec-10-enoico (HODA).
- Los hidroxidienos son plausibles intermediarios in vivo para la producción de lípidos truncados oxidativamente.
Conclusiones:
- Los hidroxidienos contribuyen a la producción in vivo de gamma-hidroxialquenales citotóxicos.
- La formación de productos intermedios de dioxetano no es esencial para la fragmentación oxidativa de los PUFA.
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