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La fosfatasa Cdc14 induce la condensación del ADNr y resuelve la cohesión independiente de la cohesina durante la
Matt Sullivan1, Toru Higuchi, Vittorio L Katis
1Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom.
Cell
|May 13, 2004
Resumen
La destrucción temprana de la cohesión en la levadura en ciernes permite la segregación cromosómica, pero el locus del ADNr requiere la fosfatasa Cdc14 para resolver los enredos y condensarse para una separación adecuada.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- La segregación cromosómica es crucial para la división celular.
- La proteasa separa la cohesina para iniciar la segregación.
- El locus del ADNr en el cromosoma XII presenta desafíos únicos de segregación.
Objetivo del estudio:
- Para investigar el papel de la destrucción de la cohesina y Cdc14 en la segregación del ADNr.
- Comprender los mecanismos que rigen la resolución del ADNr enredado.
- Para determinar cómo se ordena temporalmente la segregación del ADNr dentro del ciclo celular.
Principales métodos:
- Sistema modelo de levadura en ciernes.
- Análisis de las funciones del complejo de cohesina y condensina.
- Investigando las actividades de la separasa y la Cdc14 fosfatasa.
- Microscopia y ensayos genéticos para evaluar la condensación y segregación cromosómica.
Principales resultados:
- La destrucción de la cohesina en la metafase es insuficiente para la completa segregación del ADNr.
- El locus del ADNr permanece subcondensado y enredado en la metafase.
- Cdc14, activado por la separasa, promueve la condensación de rDNA a través de la condensina y la aurora B quinasa.
- Cdc14 también resuelve los enlaces de ADNr independientes de la cohesión, independientemente de la condensación.
Conclusiones:
- Cdc14 juega un doble papel para asegurar la segregación del ADNr.
- La condensación y la resolución de enlaces por Cdc14 son esenciales para resolver el entrelazamiento del ADNr.
- Los procesos mediados por Cdc14 establecen un orden temporal para la segregación de ADNr durante el inicio de la anafase.
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