Video Experimental Relacionado

Updated: Jun 19, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Bases estructurales para el reconocimiento específico de ARN pequeño interferente por el dominio PAZ

Jin-Biao Ma¹, Keqiong Ye¹, Dinshaw J Patel¹

¹Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York 10021, USA.

|May 21, 2004

Resumen

La unión final del ARN pequeño interferente (siRNA) por el dominio PAZ es crucial para el silenciamiento del ARN. Este estudio estructural revela cómo la PAZ ancla los dúplex de siRNA, facilitando las vías de silenciamiento de genes.

Área de la Ciencia:

Biología Molecular Biología Molecular
Biología Estructural Biología estructural.
La bioquímica es la bioquímica.

Sus antecedentes:

Los ARN cortos regulan el silenciamiento de los genes, lo que afecta la resistencia y el desarrollo del virus.
El silenciamiento del ARN implica un pequeño ARN interferente (siRNA) procesado por Dicer.
Las proteínas argonautas se unen al siRNA para formar complejos silenciadores.

Objetivo del estudio:

Determinar la estructura cristalina del dominio PAZ argonauta humano unido al siRNA.
Elucidar el mecanismo de unión del siRNA por el dominio PAZ.

Principales métodos:

Cristalografía de rayos X (2.6 A de resolución)
Pruebas de enlace bioquímico.

Principales resultados:

El dominio de la PAZ se une a un dúplex de 9-mer siRNA-como de una manera independiente de la secuencia.
La PAZ ancla el saliente 3' del 2 nucleótido y se une a la columna vertebral del fosfodiéster.
El dominio PAZ limita el residuo 5'-terminal de la hebra complementaria.

Conclusiones:

La PAZ actúa como un módulo de unión de extremo de siRNA para la transferencia en el silenciamiento de ARN.
Los anclajes PAZ guían los extremos 3' del ARN dentro de los complejos de efectores silenciadores.

Más Videos Relacionados

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Videos de Conceptos Relacionados

RNA Structure

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Conservation of Protein Domains Over Different Proteins

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...

RNA Structure

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Structure

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...