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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
Published on: June 28, 2018
El carácter dinámico del elemento G-cuadruplex en el promotor c-MYC y la modificación por TMPyP4
Jeyaprakashnarayanan Seenisamy1, Evonne M Rezler, Tiffanie J Powell
1College of Pharmacy, The University of Arizona, 1703 East Mabel, Tucson, Arizona 85721, USA.
Journal of the American Chemical Society
|July 15, 2004
Resumen
El gen c-MYC.
Área de la Ciencia:
- Biología molecular La biología molecular.
- Genética La genética.
- La bioquímica es la bioquímica.
Sus antecedentes:
- El elemento de hipersensibilidad de la nucleasa III1 (NHE III1) regula la transcripción c-MYC.
- Una estructura G-cuadruplex rica en purina dentro de NHE III1 actúa como un silenciador promotor.
Objetivo del estudio:
- Para investigar la estructura G-cuadruplex en el c-MYC NHE III1.1.
- Para determinar cómo la estructura G-cuadruplex interactúa con TMPyP4.4.
Principales métodos:
- G-cuadruplex análisis de la estructura de análisis.
- Estudios de interacción con la porfirina catiónica TMPyP4
Principales resultados:
- El c-MYC G-quadruplex forma cuatro isómeros de bucle paralelo biológicamente relevantes.
- La unión de TMPyP4 induce una conversión a estructuras mixtas paralelas / antiparalelas G-cuadruplex.
Conclusiones:
- La estructura G-cuadruplex en c-MYC NHE III1 es dinámica desde el punto de vista de la conformación.
- La unión a TMPyP4 altera la conformación G-cuadruplex, lo que afecta la regulación de los genes.
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