Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Generation of Straight or Branched Actin Filaments

Generation of Straight or Branched Actin Filaments

The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...

The Supercomplexes in the Crista Membrane

The Supercomplexes in the Crista Membrane

The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...

Porin Insertion in the Outer Mitochondrial Membrane

Porin Insertion in the Outer Mitochondrial Membrane

Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...

Structure of Porins

Structure of Porins

Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel precursors...

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

Micelles

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

An emergent disease-associated motor neuron state precedes cell death in ALS.

Cell·2026

Same author

Mutant SOD1 expressed by oligodendrocytes aggregates in myelinic nanochannels and accelerates disease progression in familial ALS mice.

bioRxiv : the preprint server for biology·2026

Same author

Short RNA chaperones promote aggregation-resistant TDP-43 conformers to mitigate neurodegeneration.

Science (New York, N.Y.)·2026

Same author

Statins and genetic inhibition of the mevalonate pathway activate an ATF3-STMN2 regenerative program.

bioRxiv : the preprint server for biology·2026

Same author

Protein-only centromeric chromatin assembly streamlines human artificial chromosome formation.

bioRxiv : the preprint server for biology·2026

Same author

Human CRAMP1 specifically promotes the expression of histone H1 genes.

EMBO reports·2026

Same journal

Keep the Hubble and James Webb Space Telescopes alive - the science is worth the price tag.

Nature·2026

Same journal

Say hello to hard helium.

Nature·2026

Same journal

How to avoid dementia - what the science really says.

Nature·2026

Same journal

Save Hubble: the race to preserve the space telescope kicks off.

Nature·2026

Same journal

How long can humans live? All evidence points to a maximum of 125 years.

Nature·2026

Same journal

Listen to Gen Z when it comes to AI in education.

Nature·2026

Ver todos los artículos relacionados

Search research articles

Video Experimental Relacionado

Updated: Jun 18, 2026

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

Determinantes estructurales para la generación de cromatina centromérica.

Ben E Black¹, Daniel R Foltz, Srinivas Chakravarthy

¹Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093, USA.

|July 30, 2004

Resumen

Este resumen es generado por máquina.

La identidad del centrómero es mantenida por una variante única de histona, CENP-A (proteína A del centrómero), que forma nucleosomas rígidos. Esta propiedad estructural, conferida por el CENP-A.

Más Videos Relacionados

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1

Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1

Published on: July 11, 2017

Videos de Experimentos Relacionados

Last Updated: Jun 18, 2026

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1

Cardiac Muscle-cell Based Actuator and Self-stabilizing Biorobot - PART 1

Published on: July 11, 2017

Área de la Ciencia:

Biología celular Biología celular.
La epigenética es la epigenética.
Biología cromosómica Biología cromosómica.

Sus antecedentes:

Los centrómeros, cruciales para la segregación cromosómica, carecen de secuencias de ADN definidas en los mamíferos.
Los centrómeros funcionales se caracterizan por la presencia de CENP-A (proteína del centrómero A), una variante de la histona H3.

Objetivo del estudio:

Investigar las propiedades estructurales del CENP-A que contiene nucleosomas.
Identificar la base estructural para la orientación y función del centrómero del CENP-A.

Principales métodos:

Intercambio de deuterio / espectrometría de masas de deuterio
Las medidas hidrodinámicas son medidas hidrodinámicas.
Experimentos de sustitución de variantes de histona en los experimentos de sustitución de variantes de histona.

Principales resultados:

El CENP-A y la histona H4 forman tetrameros subnucleosómicos más compactos y rígidos en comparación con los tetrameros H3/H4.
Un dominio específico en CENP-A es responsable de esta compactación y rigidez.
Sustituir este dominio en histona H3 es suficiente para dirigirlo a los centrómeros.

Conclusiones:

La rigidez estructural única de los nucleosomas CENP-A es conferida por un dominio específico de orientación del centromero.
Esta rigidez probablemente juega un papel crítico en el establecimiento y mantenimiento de la identidad del centrómero.
Las propiedades estructurales de CENP-A son clave para su función de garantizar la correcta segregación cromosómica.