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Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
Published on: March 18, 2014
Regulación independiente del tamaño y la actividad sináptica por el complejo promotor de la anafase
Peter van Roessel1, David A Elliott, Iain M Robinson
1Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Anatomy, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom.
Cell
|November 20, 2004
Resumen
El complejo promotor de anafase/ciclosoma (APC/C) regula el crecimiento sináptico y la transmisión en las neuronas. Esta E3 ubiquitina ligasa controla el tamaño sináptico y los niveles de receptores de glutamato en las células postmitóticas.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La plasticidad neuronal depende de la regulación precisa de las proteínas sinápticas.
- El sistema ubiquitina-proteasoma controla los niveles de proteínas, pero su papel en la ubicuidad neuronal no está claro.
- El complejo promotor de anafase / ciclosoma (APC / C) es un regulador clave del ciclo celular y la E3 ubiquitina ligasa.
Objetivo del estudio:
- Investigar el papel funcional de la APC/C en las neuronas postmitóticas.
- Para aclarar los mecanismos por los cuales el APC / C regula el desarrollo y la función sináptica.
Principales métodos:
- Utilizó Drosophila melanogaster como organismo modelo.
- Investigó el papel de la APC/C en las sinapsis neuromusculares.
- Efectores intermedios examinados incluidos los receptores de Liprin-alfa y glutamato.
Principales resultados:
- Se identificó una nueva función postmitótica para el APC/C en las sinapsis neuromusculares de Drosophila.
- Se ha demostrado la regulación dependiente de APC / C del crecimiento sináptico a través de Liprin-alfa en las neuronas.
- Se demostró el control de la transmisión sináptica mediado por APC / C mediante la regulación de los receptores de glutamato postsinápticos en los músculos.
Conclusiones:
- El APC/C desempeña funciones distintas en la regulación del crecimiento sináptico y la transmisión en las neuronas posmitóticas.
- APC/C controla el tamaño y la función sináptica a través de objetivos específicos en las neuronas y los músculos.
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