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Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Published on: July 26, 2017
Las células musculares de los mamíferos llevan una memoria celular autónoma y hereditaria de su posición rostrocaudal
M J Donoghue1, R Morris-Valero, Y R Johnson
1Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Cell
|April 3, 1992
Resumen
Las células musculares conservan la memoria posicional, con una mayor expresión de la cloramfenicol acetiltransferasa (CAT) en más músculos caudales. Esta información posicional se establece durante el desarrollo embrionario y se mantiene durante toda la edad adulta.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
- Genética La genética.
Sus antecedentes:
- Existe un gradiente rostrocaudal significativo de la expresión de la cloramfenicol acetiltransferasa (CAT) en los músculos de ratones adultos.
- Este gradiente indica una diferencia posicional en la expresión génica a lo largo del eje muscular.
Objetivo del estudio:
- Para investigar el desarrollo y mantenimiento de la información posicional en las células musculares.
- Para determinar si los mioblastos retienen su memoria posicional in vitro.
Principales métodos:
- El establecimiento de cultivos celulares a partir de músculos de ratón adulto.
- La obtención de clones a partir de células miogénicas (satélites) adultas individuales.
- Cultivo de mioblastos embrionarios y líneas celulares satélite transformadas por oncogenes.
Principales resultados:
- Los niveles de CAT in vitro reflejaron con precisión la posición rostrocaudal del músculo de origen.
- La memoria posicional se observó en cultivos de músculos adultos, mioblastos embrionarios y líneas celulares.
- Esta memoria era autónoma de la célula, heredable y estable.
Conclusiones:
- Los mioblastos poseen una memoria posicional intrínseca establecida durante la embriogénesis.
- Esta memoria se mantiene en las células adultas y es estable a la transformación.
- Las líneas celulares clonales proporcionan un modelo valioso para estudiar esta memoria posicional.
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