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Analytical Techniques for Assaying Nitric Oxide Bioactivity

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Control de la bioactividad plasmática del óxido nítrico por los perfluorocarbonos: mecanismos fisiológicos e

Olga Rafikova¹, Elena Sokolova, Ruslan Rafikov

¹Department of Biochemistry, New York University Medical Center,New York, NY 10016, USA.

|November 24, 2004

Resumen

Las emulsiones de perfluorocarbonos (PFC) absorben y transportan óxido nítrico (NO), catalizando su conversión en compuestos vasoactivos. Esto explica los efectos hemodinámicos de PFC y sugiere su uso en el tratamiento de problemas cardiovasculares relacionados con NO.

Área de la Ciencia:

Ingeniería Biomédica Ingeniería Biomédica.
Fisiología cardiovascular fisiología cardiovascular.
Farmacología Farmacología.

Sus antecedentes:

Los perfluorocarbonos (PFC) son portadores de oxígeno inertes con potencial como sustitutos de la sangre.
Las emulsiones de PFC demuestran una capacidad inesperada para absorber y transportar óxido nítrico (NO).

Objetivo del estudio:

Para investigar la interacción de las emulsiones de PFC con el óxido nítrico (NO).
Para aclarar el mecanismo de la transformación de NO mediada por PFC y sus consecuencias fisiológicas.

Principales métodos:

Detección electroquímica de productos de oxidación de NO y S-nitrosotiolos (RSNO) in vitro e in vivo.
Administración de diferentes concentraciones de PFC y tiolos exógenos a ratas para evaluar las respuestas hemodinámicas.

Principales resultados:

Los PFC catalizan la oxidación de NO dentro de las micelas, formando S-nitrosotiolos vasoactivos (RSNO).
La formación óptima de RSNO se produjo a ~ 1% de concentración de PFC; concentraciones más altas actuaron como sumideros de NO.
Las dosis bajas de PFC indujeron hipotensión, potenciada por los tiolos, mientras que las dosis altas causaron hipertensión.

Conclusiones:

Las emulsiones de PFC modulan el NO endógeno plasmático, influyendo en su bioactividad.
Los PFC representan un nuevo enfoque farmacológico para las afecciones cardiovasculares relacionadas con el desequilibrio de NO.

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