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Videos de Conceptos Relacionados

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Published on: June 15, 2016

El factor de transcripción de moléculas pequeñas imita el factor de transcripción.

Youngjoo Kwon1, Hans-Dieter Arndt, Qian Mao

  • 1The Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.

Journal of the American Chemical Society
|December 9, 2004
PubMed
Resumen

Los investigadores crearon una molécula sintética no peptídica que imita un factor de transcripción. Este nuevo compuesto controla la expresión génica al unirse al ADN y a un complejo proteico, lo que demuestra un nuevo enfoque en biología química.

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Área de la Ciencia:

  • Biología Química Biología química.
  • Biología Molecular Biología Molecular
  • Química sintética de la química sintética.

Sus antecedentes:

  • Los factores de transcripción regulan la expresión génica, un proceso fundamental en la biología eucariota.
  • El control externo de los factores de transcripción utilizando pequeñas moléculas orgánicas es un desafío significativo en la química.

Objetivo del estudio:

  • Diseñar y sintetizar un compuesto completamente orgánico y no peptídico que imite la función de un factor de transcripción.
  • Para demostrar que tal molécula sintética puede controlar externamente la expresión génica.

Principales métodos:

  • Diseñó una molécula híbrida que combina una poliamida de horquilla para la unión al ADN y wrenchnolol para la unión a la subunidad Sur-2 del complejo mediador humano.
  • Probó la capacidad del compuesto para activar la transcripción de un gen reportero in vitro.

Principales resultados:

  • El compuesto orgánico híbrido activó con éxito la transcripción del gen reportero de una manera dependiente del promotor.
  • El compuesto logró esta activación a través de interacciones simultáneas tanto con el ADN como con la proteína Sur-2.

Conclusiones:

  • Es factible diseñar moléculas sintéticas no peptídicas que funcionen como factores de transcripción.
  • Este trabajo abre nuevas vías para controlar la expresión génica utilizando compuestos orgánicos diseñados.