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El cofolding organiza el ARN del virus del mosaico de alfalfa y la proteína de la capa para la replicación
Laura M Guogas1, David J Filman, James M Hogle
1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
Science (New York, N.Y.)
|December 18, 2004
Resumen
El virus del mosaico de alfalfa requiere que su proteína de capa se una al ARN 3' termini para la infectividad. El análisis estructural revela interacciones de ARN-proteína conservadas cruciales para la replicación viral.
Área de la Ciencia:
- Virología Virología.
- Biología Estructural Biología estructural.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La infectividad del virus del mosaico de la alfalfa (AIMV) depende de la interacción entre sus ARN genómicos y la proteína de la capa viral.
- La proteína de la capa debe unirse a los 3 'terminales de los ARN virales para la infectividad.
Objetivo del estudio:
- Aclarar la base estructural de la interacción entre la proteína de la capa del AIMV y su ARN genómico 3' termini.
- Para entender cómo esta interacción facilita la infectividad viral y la replicación.
Principales métodos:
- Determinación de la estructura cristalina de un virus mosaico de alfalfa del complejo ARN-péptido.
- Análisis de secuencias de ARN conservadas (repeticiones AUGC) y motivos de aminoácidos de proteína de capa (Pro-Thr-x-Arg-Ser-x-x-Tyr).
Principales resultados:
- La estructura cristalina reveló un acoplamiento específico de repeticiones AUGC conservadas y aminoácidos de proteína de capa AIMV.
- Los residuos alternos de AUGC exhiben orientaciones opuestas y pares de bases en dúplex adyacentes.
- Las inversiones de la columna vertebral del ARN, estabilizadas por las interacciones arginina-guanina, dan como resultado un ordenamiento específico de nucleótidos.
Conclusiones:
- El estudio sugiere que una conformación de ARN 3' uniforme y organizada es crítica para la replicación del AIMV.
- Esta conformación se asemeja a la de los ARN virales con extremos similares al ARN de transferencia.
- Las interacciones específicas ARN-proteína que involucran elementos conservados son clave para la infectividad viral.
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