Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Feedback Inhibition00:46

Feedback Inhibition

44.2K
Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
44.2K
The Electron Transport Chain01:30

The Electron Transport Chain

13.9K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
13.9K
Mitochondrial Protein Sorting01:39

Mitochondrial Protein Sorting

4.4K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death.  Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.4K
Mitochondrial Precursor Proteins01:39

Mitochondrial Precursor Proteins

2.9K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
2.9K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

8.9K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
8.9K
Energy to Drive Translocation01:37

Energy to Drive Translocation

2.0K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.0K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Impact of ultra-low tidal volume ventilation on 1-year functional outcome in COVID-19 ARDS patients. A long-term follow-up analysis of a randomized controlled trial.

Critical care (London, England)·2026
Same author

Outcomes, prognostic factors, and the role of intracranial pressure monitoring in severe community-acquired bacterial meningitis: a multicenter retrospective cohort study.

The Lancet regional health. Europe·2026
Same author

HydrOcortisone and fludRocortisoNe for critical ILLness-related corticosteroid insufficiency (HORNbILL): study protocol for a multicentre randomised placebo-controlled trial.

BMJ open·2026
Same author

Monitoring T-cell function in septic shock: one-year experience with a fully automated assay.

Critical care (London, England)·2026
Same author

Potentially Surgical Digestive Complications in Patients With Status Epilepticus: Insights From the ICTAL Registry.

Critical care medicine·2026
Same author

Low versus standard calorie and protein feeding and renal dysfunction in ventilated adults with shock: a NUTRIREA-3 post hoc analysis.

Intensive care medicine·2026

Video Experimental Relacionado

Updated: May 6, 2026

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
13:42

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Published on: September 7, 2012

20.9K

El postcondicionamiento inhibe la transición de la permeabilidad mitocondrial.

Laurent Argaud1, Odile Gateau-Roesch, Olivier Raisky

  • 1INSERM E 0226, Université Claude Bernard Lyon I, France.

Circulation
|January 12, 2005
PubMed
Resumen

El postcondicionamiento, una breve intervención durante la reperfusión, reduce significativamente el tamaño del ataque cardíaco al inhibir el poro de transición de permeabilidad mitocondrial (mPTP). Este efecto protector ofrece poderosos beneficios antiisquémicos.

Más Videos Relacionados

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
08:43

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

18.6K
Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess
07:35

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess

Published on: June 1, 2022

1.9K

Videos de Experimentos Relacionados

Last Updated: May 6, 2026

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
13:42

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Published on: September 7, 2012

20.9K
Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
08:43

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

18.6K
Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess
07:35

Assessment of Open Probability of the Mitochondrial Permeability Transition Pore in the Setting of Coenzyme Q Excess

Published on: June 1, 2022

1.9K

Área de la Ciencia:

  • Investigaciones Cardiovasculares Investigación Cardiovascular Investigaciones Cardiovasculares
  • Biología mitocondrial Biología mitocondrial
  • Lesiones isquémicas por lesión isquémica.

Sus antecedentes:

  • La isquemia breve durante la reperfusión, conocida como postcondicionamiento, puede limitar el tamaño del infarto.
  • La apertura del poro de transición de permeabilidad mitocondrial (mPTP) está implicada en lesiones letales por reperfusión.
  • El estudio investigó si el postcondicionamiento influye en la apertura de mPTP.

Objetivo del estudio:

  • Para determinar si el postcondicionamiento modula la apertura del poro de la transición de permeabilidad mitocondrial (mPTP).
  • Evaluar los efectos protectores del postcondicionamiento contra la lesión por isquemia-reperfusión.

Principales métodos:

  • Los conejos de pecho abierto se sometieron a 30 minutos de isquemia y 4 horas de reperfusión.
  • El postcondicionamiento implicó breves ciclos de isquemia y reperfusión después del reflujo inicial.
  • Se midieron la apertura de mPTP inducida por Ca2+ mitocondrial y el tamaño del infarto.

Principales resultados:

  • El postcondicionamiento, el precondicionamiento y el inhibidor de mPTP NIM811 redujeron significativamente el tamaño del infarto en comparación con los controles (29%, 18%, 20% vs. 61%).
  • La carga de Ca2+ requerida para abrir la mPTP fue significativamente mayor en los grupos postcondicionados, precondicionados y tratados con NIM811 (41, 47, 67 μmol/L CaCl2/mg) frente a los controles (16 μmol/L CaCl2/mg).

Conclusiones:

  • El postcondicionamiento inhibe efectivamente la apertura del poro de transición de permeabilidad mitocondrial (mPTP).
  • Esta inhibición confiere una protección antiisquémica significativa, reduciendo el tamaño del infarto.