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La actividad de chaperón de la proteína O-fucosiltransferasa 1 promueve el plegamiento del receptor de muesca
Tetsuya Okajima1, Aiguo Xu, Liang Lei
1Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA.
Science (New York, N.Y.)
|February 5, 2005
Resumen
La proteína O-fucosiltransferasa 1 (OFUT1) actúa como un acompañante para los receptores de Notch, ayudando a su plegamiento y transporte. Esta es la glicosiltransferasa.
Área de la Ciencia:
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
- Biología del desarrollo Biología del desarrollo.
Sus antecedentes:
- La vía de señalización de la muesca regula las decisiones del destino celular.
- La proteína O-fucosiltransferasa 1 (OFUT1) glicosilados Receptores de muesca.
Objetivo del estudio:
- Investigar el papel de OFUT1 más allá de su actividad enzimática.
- Determina si OFUT1 tiene una función de acompañante para Notch.
Principales métodos:
- Estudió la localización de OFUT1 y la unión a Notch en Drosophila.
- Evaluó el efecto de OFUT1 en el tráfico y plegado de Notch.
- Rescate examinado de mutaciones de Notch por OFUT1.1.
Principales resultados:
- OFUT1 se localiza en el retículo endoplasmático y se une a Notch.
- OFUT1 es esencial para el transporte de Notch desde el retículo endoplasmático.
- OFUT1 facilita el plegamiento de Notch independientemente de su actividad de glucosiltransferasa.
Conclusiones:
- OFUT1 posee una nueva función de acompañante para Notch.
- Las glicosiltransferasas pueden actuar como acompañantes en el retículo endoplasmático.
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