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Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
Published on: January 20, 2017
La activación de una proteína efector bacteriana fitopatógena por una ciclofilina eucariota
Gitta Coaker1, Arnold Falick, Brian Staskawicz
1Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
Science (New York, N.Y.)
|March 5, 2005
Resumen
El receptor inmunológico vegetal RPS2 utiliza una proteína efector bacteriana, AvrRpt2, para activar la defensa. AvrRpt2, activado por la ciclofilina, divide RIN4, iniciando la resistencia de las plantas contra Pseudomonas syringae.
Área de la Ciencia:
- Inmunidad vegetal La inmunidad vegetal.
- Interacciones moleculares entre plantas y microbios.
- La patogénesis bacteriana es la patogénesis bacteriana.
Sus antecedentes:
- Las plantas superiores poseen inmunidad innata para detectar las proteínas efectoras bacterianas.
- La resistencia de Arabidopsis a Pseudomonas syringae se basa en la proteína RPS2 cuando están presentes efectores como AvrRpt2.
Objetivo del estudio:
- Para aclarar el mecanismo por el cual AvrRpt2 activa la inmunidad vegetal mediada por RPS2.
- Para identificar el factor huésped responsable de la activación de AvrRpt2.
Principales métodos:
- Investigó el papel de AvrRpt2 en la señalización de defensa de plantas.
- Identificó el factor huésped que activa la actividad de la proteasa de AvrRpt2.
- Determinado el objetivo de AvrRpt2 dentro de la célula vegetal.
Principales resultados:
- AvrRpt2 es una proteasa de cisteína que divide la proteína RIN4 de la Arabidopsis.
- La escisión de RIN4 por AvrRpt2 actúa como una señal para la resistencia mediada por RPS2.
- Un factor eucariótico, identificado como ciclofilina, es necesario para la activación de AvrRpt2 y la subsiguiente escisión de RIN4.
Conclusiones:
- La activación mediada por ciclofilina de AvrRpt2 es esencial para su actividad de proteasa.
- El AvrRpt2 activo divide directamente el RIN4, desencadenando respuestas de defensa de la planta.
- Esta vía pone de relieve un sofisticado mecanismo de vigilancia de las plantas contra patógenos bacterianos.
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