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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
(SR/RS) - PNA de ciclohexanilo: análogos de PNA preorganizados conformacionalmente con una preferencia sin
T Govindaraju1, Vaijayanti A Kumar, Krishna N Ganesh
1Organic Chemistry Division (Synthesis), National Chemical Laboratory, Pune 411008, India.
Journal of the American Chemical Society
|March 24, 2005
Resumen
Los oligómeros de ácido nucleico peptídico modificado (PNA) con ciclohexil-timina mostraron una mayor unión al ARN sobre el ADN. Los oligómeros de ciclopentilo-timina PNA se unen igualmente bien tanto al ARN como al ADN.
Área de la Ciencia:
- Química Medicinal La Química Medicinal es el campo de la Química Medicinal.
- La bioquímica es la bioquímica.
- Química del oligonucleótido Química del oligonucleótido
Sus antecedentes:
- Los ácidos nucleicos péptidos (PNA) son imitaciones de ADN con una columna vertebral neutral.
- Las modificaciones en la columna vertebral del PNA pueden alterar la afinidad de unión y la selectividad para los objetivos de ácido nucleico.
Objetivo del estudio:
- Para investigar el efecto de las modificaciones de ciclohexilo y ciclopentilo en las bases de timina PNA en la estabilidad de duplex con ARN y ADN.
- Para determinar si estas modificaciones introducen selectividad para la unión de ARN o ADN.
Principales métodos:
- Síntesis de oligómeros de PNA que contienen cis-(1S,2R/1R,2S) -ciclohexil-timina (III) y cis-(1S,2R/1R,2S) -ciclopentil-timina (IV).
- Formación y desnaturalización térmica (análisis TM) de los dúplex de PNA: ARN y PNA: ADN.
Principales resultados:
- Los oligómeros de PNA con ciclohexil-timina formaron dúplex de PNA:ARN con temperaturas de fusión (Tm) 30-50°C más altas que los dúplex de PNA:ADN, lo que indica una mayor afinidad por el ARN.
- Los oligómeros de PNA con ciclopentilo-timina formaron duplexos altamente estables con ARN y ADN sin discriminación significativa.
Conclusiones:
- La modificación de ciclohexilo de la timina PNA puede conferir selectividad para la unión al ARN.
- La modificación ciclopentílica de la timina PNA da como resultado una unión no discriminatoria y de alta afinidad tanto al ARN como al ADN.
- Estos PNA modificados ofrecen potencial para aplicaciones que requieren interacciones específicas o amplias de ácido nucleico.
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